In vitro antifungal resistance and molecular analysis of the SQLE gene in dermatophytosis: a laboratory-based study.

IF 1.9 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2026-06-01 DOI:10.18502/ijm.v18i3.21667

Jegan Charlies, Latha Ragunathan, Kavitha Kanniyan, Jeyakumari Duraipandian

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引用次数: 0

Abstract

Background and objectives: Dermatophytosis is a common superficial fungal infection that affects the keratinised tissues. This study examines the antifungal resistance mechanisms and molecular detection of squalene epoxidase gene alterations in clinical isolates from patients.

Materials and methods: A cross-sectional study conducted from January 2019 to December 2020 included 110 clinically suspected dermatophytosis specimens. Microscopy, culture, CLSI M38-A2 broth microdilution testing, and PCR-based ITS and SQLE (520 bp) amplification were performed. MIC50, MIC90, geometric mean, and ranges were analysed using SPSS version 19 with P < 0.05.

Results: A total of 110 suspected cases were evaluated, with 78 (70.9%) males. KOH positivity reached 100%, while culture positivity was 47.3% (52/110). Trichophyton rubrum accounted for 38.5% isolates. Fluconazole MICs were ≥64 μg/mL. 39/52 isolates showed terbinafine resistance, with 23 expressing the 520 bp SQLE gene.

Conclusion: Terbinafine and caspofungin showed consistent in vitro activity, while fluconazole showed limited activity. Routine susceptibility testing guides drug selection, improves treatment outcomes, and supports rational management of resistant dermatophytosis cases.

查看原文本刊更多论文

皮肤癣SQLE基因的体外抗真菌抗性和分子分析：基于实验室的研究。

背景和目的：皮肤真菌病是一种常见的影响角质组织的浅表真菌感染。本研究探讨临床分离患者角鲨烯环氧化酶基因改变的抗真菌耐药机制和分子检测。材料与方法：2019年1月至2020年12月横断面研究纳入110例临床疑似皮肤癣标本。显微镜、培养、CLSI M38-A2肉汤微量稀释试验、基于pcr的ITS和SQLE （520 bp）扩增。MIC50、MIC90、几何平均值、极差采用SPSS 19分析，P < 0.05。结果：共检出疑似病例110例，其中男性78例，占70.9%。KOH阳性为100%，培养阳性为47.3%（52/110）。红毛癣菌占38.5%。氟康唑mic≥64 μg/mL。39/52株对特比萘芬耐药，其中23株表达520bp的SQLE基因。结论：特比萘芬与卡泊芬金体外活性一致，氟康唑体外活性有限。常规药敏试验指导药物选择，改善治疗效果，并支持耐药皮肤真菌病病例的合理管理。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.