{"title":"Carvotanacetone from Saposhnikoviae radix restores skin barrier by regulating SP1 and Claudin-1.","authors":"Liying Jia, Weihong Ying, Gairong Liu, Peng Yang, Yizhi Zheng","doi":"10.4149/gpb_2026005","DOIUrl":null,"url":null,"abstract":"<p><p>This study aimed to investigate how Saposhnikoviae radix regulates skin barrier function. Bioinformatics analysis was conducted to identify the compounds in Saposhnikoviae radix and their target genes related to cell barrier function. Molecular docking analysis was performed to investigate the interaction between carvotanacetone (CARV) and key genes. The impact of CARV on skin barrier function was assessed in HaCaT cells treated with tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ). The effects of CARV and specificity protein 1 (SP1) on cell viability and permeability were analyzed. CARV effectively interacted with SP1. CARV attenuated the TNF-α/IFN-γ induced impairment of cell viability and Claudin-1 expression in an SP1-dependent manner, as SP1 knockdown abrogated its beneficial effects on Claudin-1 and cell permeability. CARV, a compound derived from Saposhnikoviae radix, protects HaCaT cells from barrier dysfunction by upregulating Claudin-1 expression via the SP1.</p>","PeriodicalId":12514,"journal":{"name":"General physiology and biophysics","volume":"45 3","pages":"259-268"},"PeriodicalIF":1.5000,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"General physiology and biophysics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.4149/gpb_2026005","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
This study aimed to investigate how Saposhnikoviae radix regulates skin barrier function. Bioinformatics analysis was conducted to identify the compounds in Saposhnikoviae radix and their target genes related to cell barrier function. Molecular docking analysis was performed to investigate the interaction between carvotanacetone (CARV) and key genes. The impact of CARV on skin barrier function was assessed in HaCaT cells treated with tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ). The effects of CARV and specificity protein 1 (SP1) on cell viability and permeability were analyzed. CARV effectively interacted with SP1. CARV attenuated the TNF-α/IFN-γ induced impairment of cell viability and Claudin-1 expression in an SP1-dependent manner, as SP1 knockdown abrogated its beneficial effects on Claudin-1 and cell permeability. CARV, a compound derived from Saposhnikoviae radix, protects HaCaT cells from barrier dysfunction by upregulating Claudin-1 expression via the SP1.
期刊介绍：
General Physiology and Biophysics is devoted to the publication of original research papers concerned with general physiology, biophysics and biochemistry at the cellular and molecular level and is published quarterly by the Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences.
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