Hang Tian, Zhiying Cui, Zhiyou Lv, Mengqiu Liu, Yang Liu, Yilin Zhao, Weiyan Yin, Mengru Zhao, Hongyan Yu, Mengchao Zhou, Yaxian Lu, Dapeng Li, Ming Gong, Haitao Xu, Zhijun Hou
{"title":"Molecular detection and zoonotic genotyping of Enterocytozoon bieneusi in Siberian tigers (Panthera tigris altaica), Northeast China.","authors":"Hang Tian, Zhiying Cui, Zhiyou Lv, Mengqiu Liu, Yang Liu, Yilin Zhao, Weiyan Yin, Mengru Zhao, Hongyan Yu, Mengchao Zhou, Yaxian Lu, Dapeng Li, Ming Gong, Haitao Xu, Zhijun Hou","doi":"10.1007/s00436-026-08715-0","DOIUrl":null,"url":null,"abstract":"<p><p>Enterocytozoon bieneusi is an important zoonotic pathogen. This study examined its prevalence and genotypes in Siberian tigers in Northeast China. Fecal samples from 97 captive and 5 wild tigers were analyzed by nested PCR targeting the ITS region. Prevalence in captive tigers was 40.2% (39/97), with no significant site differences. Three genotypes were identified: D (89.7%), Peru8 (7.7%), and Type IV (2.6%), all within zoonotic Group 1. E. bieneusi was detected for the first time in a wild Siberian tiger (genotype D). The predominance of genotype D-common in poultry-suggests dietary transmission through contaminated chicken meat. These results indicate high prevalence of zoonotic genotypes in tigers, underscoring cross-species transmission risks and the need for strengthened biosecurity and surveillance.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":" ","pages":""},"PeriodicalIF":1.9000,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13506582/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Parasitology Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s00436-026-08715-0","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PARASITOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Enterocytozoon bieneusi is an important zoonotic pathogen. This study examined its prevalence and genotypes in Siberian tigers in Northeast China. Fecal samples from 97 captive and 5 wild tigers were analyzed by nested PCR targeting the ITS region. Prevalence in captive tigers was 40.2% (39/97), with no significant site differences. Three genotypes were identified: D (89.7%), Peru8 (7.7%), and Type IV (2.6%), all within zoonotic Group 1. E. bieneusi was detected for the first time in a wild Siberian tiger (genotype D). The predominance of genotype D-common in poultry-suggests dietary transmission through contaminated chicken meat. These results indicate high prevalence of zoonotic genotypes in tigers, underscoring cross-species transmission risks and the need for strengthened biosecurity and surveillance.
期刊介绍：
The journal Parasitology Research covers the latest developments in parasitology across a variety of disciplines, including biology, medicine and veterinary medicine. Among many topics discussed are chemotherapy and control of parasitic disease, and the relationship of host and parasite.
Other coverage includes: Protozoology, Helminthology, Entomology; Morphology (incl. Pathomorphology, Ultrastructure); Biochemistry, Physiology including Pathophysiology;
Parasite-Host-Relationships including Immunology and Host Specificity; life history, ecology and epidemiology; and Diagnosis, Chemotherapy and Control of Parasitic Diseases.
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