The antibacterialactivity of Barangan (Musa acuminata Colla) peel on Streptococcus mutans: In silico and in vitro study.

Abstract

This study aims to evaluate the bacterial activity of Barangan peel against Streptococcus mutans (S. mutans) using in silico and in vitro methods. In silico conducted with AutoDock 4.0 evaluated ligand binding to GtfB (PDB ID: 3AIB), SwissADME, ProTox-II, and PASS online assessed pharmacokinetic, toxicity, and biological activity profiles. Antibacterial activity of Barangan peel at 10%, 20%, 35%, and 50% concentrations against S. mutans was determined using agar assays. The inhibition zones and colony count data were analyzed using one-way ANOVA (p≤0.05). Results showed Barangan peel phytochemical has low toxicity (Class 5-6, LD50 >2,000 mg/kg) and high biological potential for non-phenolic terpenoids (Pa=0.405). Cyclopropa stigmat steroid has the strongest affinities (-8.00 kcal/mol). The minimum inhibitory concentration (MIC) was at a 10% concentration, while the inhibition zone diameter and colony count showed significant differences (p<0.05) for all concentrations. Barangan peel phytochemicals exhibited favorable toxicity profiles and potent inhibitory activity against S. mutans.