Hidden Fungal DNA Structures May Shape Sequencing Outcomes

IF 3.3 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

BioEssays Pub Date : 2026-06-15 DOI:10.1002/bies.70153

Paul W. Thomas

引用次数: 0

Abstract

Fungal DNA is systematically under-detected in shotgun metagenomics, likely due in part to physical barriers like melanized cell walls and complex DNA conformations. Additionally, Oxford Nanopore Technologies sequencing with native fungal DNA often results in rapid pore clogging and unusual translocation dynamics, possibly due to intrinsic, yet undescribed, structural complexities. Exploring these signals could reveal novel fungal genome architectures, enhance sequencing accuracy, and drive advances in fungal biology.

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隐藏的真菌DNA结构可能影响测序结果。

在散弹枪宏基因组学中，真菌DNA系统地未被检测到，部分原因可能是物理障碍，如黑化的细胞壁和复杂的DNA构象。此外，Oxford Nanopore Technologies对天然真菌DNA的测序通常会导致快速的孔隙堵塞和不寻常的移位动力学，这可能是由于内在的、尚未描述的结构复杂性。探索这些信号可以揭示新的真菌基因组结构，提高测序准确性，并推动真菌生物学的进步。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BioEssays 生物-生化与分子生物学

CiteScore

7.30

自引率

2.50%

发文量

167

审稿时长

4-8 weeks

期刊介绍： molecular – cellular – biomedical – physiology – translational research – systems - hypotheses encouraged BioEssays is a peer-reviewed, review-and-discussion journal. Our aims are to publish novel insights, forward-looking reviews and commentaries in contemporary biology with a molecular, genetic, cellular, or physiological dimension, and serve as a discussion forum for new ideas in these areas. An additional goal is to encourage transdisciplinarity and integrative biology in the context of organismal studies, systems approaches, through to ecosystems, where appropriate.