Lipidome and Targeted Metabolome Analysis of Locational Picrorhiza Kurroa Royle. Ex Benth., Using UPLC-PDA and UHPLC-QTOF-IMS

IF 1.8 3区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Rapid Communications in Mass Spectrometry Pub Date : 2026-06-14 DOI:10.1002/rcm.70108

Bindu Rawat, Robin Joshi, Dinesh Kumar

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引用次数: 0

Abstract

Introduction

Lipids are a fundamental class of biomolecules essential for membrane structure, energy storage, and cellular signaling, whereas lipidomics is an advanced dedicated way to understand the lipids, including biological lipids.

Objective

Keeping in mind the importance of lipid makeup, the current study was focused to understand the lipid diversity present within Picrorhiza kurroa (leaves, rhizomes, and roots) at three different localities of Himachal Pradesh, India: Bhatori (Pangi), Bharmaur (Chamba), and Gumna (Shimla).

Method

UHPLC-QTOF-IMS was used for lipid profiling of P. kurroa locational samples. The data were analyzed for each extraction parameter. Moreover, targeted specialized metabolites were also analyzed using UPLC-PDA. Further, multivariate analysis including supervised orthogonal projections to latent structures discriminant analysis and unsupervised principal component analysis were employed to understand the lipid makeup, its dissemination, and similarity traits among the samples.

Results

Q-TOF/MS revealed the comprehensive lipidome of P. kurroa that includes the important presence of Cer, FA, (L) PE, TG, PA, PI, PS, MGDG, and DGDG. Leaves of P. kurroa collected from the Pangi region showed higher lipid content than other targeted parts and localities, whereas targeted metabolites including picrosides were present higher in the rhizomes of the Pangi region. Further, statistical analysis showed clear dissemination and similarity traits among the samples. The current study suggested variability among the chemo-profile of different locational samples.

Conclusion

We concluded that leaves of P. kurroa collected from the Pangi region constitute higher lipid content than other parts and P. kurroa plants collected from another area. Also, the findings revealed that the plant P. kurroa contains Cer, FA, (L)PE, TG, DG, PG, PA, PI, PS, MGDG, and DGDG. The variation in picroside contents was also observed through UPLC-DAD analysis; rhizomes collected from the Bhatori (Pangi) region exhibited the highest picroside content.

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定位性黑斑病的脂质组和靶向代谢组分析。Benth交货。采用UPLC-PDA和UHPLC-QTOF-IMS。

脂质是一类基本的生物分子，对膜结构、能量储存和细胞信号传导至关重要，而脂质组学是一种先进的专用方法来了解脂质，包括生物脂质。目的：考虑到脂质组成的重要性，本研究的重点是了解在印度喜马偕尔邦三个不同地区：Bhatori (Pangi), Bharmaur （Chamba）和Gumna （Shimla）的Picrorhiza kurroa（叶，根茎和根）中存在的脂质多样性。方法：采用UHPLC-QTOF-IMS法对黑曲霉定位样品进行脂质谱分析。对每个提取参数的数据进行分析。此外，还使用UPLC-PDA分析了靶向的特化代谢物。此外，采用多变量分析，包括监督正交投影到潜在结构判别分析和无监督主成分分析，以了解样本中的脂质组成、分布和相似性特征。结果：Q-TOF/MS揭示了黑田鼠的综合脂质组，包括Cer、FA、(L) PE、TG、PA、PI、PS、MGDG和DGDG的重要存在。从Pangi地区收集的黑桫椤叶片的脂质含量高于其他目标部位和部位，而在Pangi地区的根茎中，包括微甜苷在内的目标代谢物含量更高。此外，统计分析显示样本间具有明显的传播性和相似性。目前的研究表明，不同地点样品的化学特征存在差异。结论：从Pangi地区采集的黑桫椤叶片的脂质含量高于其他部位和其他地区的黑桫椤。此外，研究结果还表明，该植物含有Cer、FA、(L)PE、TG、DG、PG、PA、PI、PS、MGDG和DGDG。UPLC-DAD分析了芍药苷含量的变化；产自Bhatori （Pangi）地区的根状茎中picro苷含量最高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Rapid Communications in Mass Spectrometry 化学-分析化学

CiteScore

4.10

自引率

5.00%

发文量

219

审稿时长

2.6 months

期刊介绍： Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.