Long-Read Sequencing and Comparative Genome Analyses of Seven Emergomyces Type-Strains.

IF 4.1 3区生物学 Q2 MYCOLOGY

Mycopathologia Pub Date : 2026-06-12 DOI:10.1007/s11046-026-01085-2

Ilan S Schwartz, Bert Gerrits van den Ende, Ferry Hagen

引用次数: 0

Abstract

Emergomyces (Ajellomycetaceae, Onygenales) is a genus of dimorphic fungal pathogens that cause severe, opportunistic infections around the world. We used long-read nanopore sequencing to generate de novo genome assemblies followed by comparative analyses for the type-strains of Emergomyces africanus, Emergomyces canadensis, Emergomyces crescens, Emergomyces europaeus, Emergomyces orientalis, Emergomyces pasteurianus, and Emergomyces soli. The average Emergomyces genome was found to be 32,965,087 bp in size (range 28,687,700-35,863,369 bp) with GC%-contents of 42.01-45.72%. Average Nucleotide Identity analysis of the seven type-strain genomes with the publicly available Emergomyces and Blastomyces genomes was performed, showing values of 85-100% similarity between genomes and 85-91% between the twelve species tested. Furthermore, we formally validate the species descriptions for Emergomyces crescens and Emergomyces soli.

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7种新兴菌型菌株的长读测序和比较基因组分析。

新兴菌属（Ajellomycetaceae, Onygenales）是一种二态真菌病原体属，在世界各地引起严重的机会性感染。我们使用长读纳米孔测序技术生成了全新的基因组组装，然后对非洲新兴菌、加拿大新兴菌、新月形新兴菌、欧洲新兴菌、东方新兴菌、巴氏新兴菌和soli新兴菌进行了比较分析。新兴菌基因组平均大小为32,965,087 bp (28,687,700 ~ 35,863,369 bp)， GC%-含量为42.01 ~ 45.72%。对7个型株基因组与公开的Emergomyces和Blastomyces基因组进行了平均核苷酸同源性分析，结果显示7个型株基因组之间的相似性为85-100%，12个种之间的相似性为85-91%。此外，我们还正式验证了新月新兴omyces crescenes和soli新兴omyces的物种描述。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Mycopathologia 生物-真菌学

CiteScore

6.80

自引率

3.60%

发文量

审稿时长

3 months

期刊介绍： Mycopathologia is an official journal of the International Union of Microbiological Societies (IUMS). Mycopathologia was founded in 1938 with the mission to ‘diffuse the understanding of fungal diseases in man and animals among mycologists’. Many of the milestones discoveries in the field of medical mycology have been communicated through the pages of this journal. Mycopathologia covers a diverse, interdisciplinary range of topics that is unique in breadth and depth. The journal publishes peer-reviewed, original articles highlighting important developments concerning medically important fungi and fungal diseases. The journal highlights important developments in fungal systematics and taxonomy, laboratory diagnosis of fungal infections, antifungal drugs, clinical presentation and treatment, and epidemiology of fungal diseases globally. Timely opinion articles, mini-reviews, and other communications are usually invited at the discretion of the editorial board. Unique case reports highlighting unprecedented progress in the diagnosis and treatment of fungal infections, are published in every issue of the journal. MycopathologiaIMAGE is another regular feature for a brief clinical report of potential interest to a mixed audience of physicians and laboratory scientists. MycopathologiaGENOME is designed for the rapid publication of new genomes of human and animal pathogenic fungi using a checklist-based, standardized format.