Innovative Spherical-Shaped Gel Electrophoresis Apparatuses for Scientific Demonstrations to High School Students

Abstract

Gel electrophoresis (GE) is the most routinely applied technique for detecting, identifying, and characterizing biomolecules. All GE instruments operate on established principles but vary in their operational features. Implementing the GE technique for education and research is considered simple due to its procedure, result interpretation, and cost-effectiveness. Published literature showed that educators are always interested in providing practical experiences to biochemistry students for polyacrylamide/agarose-related GE techniques. Modern GE instruments are sometimes considered challenging due to critical operating steps and maintenance expenses. We have developed two spherical-shaped apparatuses for vertical and horizontal GE techniques, equipped with cost-effective modern features and easily available parts/components to eliminate laborious and time-consuming steps. Both apparatuses provide easy setup, safe operation, and hassle-free operation. High school students verified the performance of our apparatuses in a short-term training program with positive outcomes. Comparisons with standard apparatuses revealed that our apparatuses are fast, user-friendly, and applicable for young researchers.