Resilience in Tribolium Castaneum Through Antioxidant Priming Under Phosphine-Induced Oxidative Stress.

IF 3.2 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biotechnology Pub Date : 2026-06-08 DOI:10.1007/s12033-026-01586-x

Mudagadde G Deeksha, Suresh M Nebapure, Doddachowdappa Sagar, Ramcharan Bhattacharya, Anil Dahuja, Sabtharishi Subramanian

{"title":"Resilience in Tribolium Castaneum Through Antioxidant Priming Under Phosphine-Induced Oxidative Stress.","authors":"Mudagadde G Deeksha, Suresh M Nebapure, Doddachowdappa Sagar, Ramcharan Bhattacharya, Anil Dahuja, Sabtharishi Subramanian","doi":"10.1007/s12033-026-01586-x","DOIUrl":null,"url":null,"abstract":"This study investigates the effects of sublethal and high-dose phosphine exposure on biocatalytic activity and gene expression of key antioxidants (catalase, peroxidase, and superoxide dismutase) in T. castaneum populations: susceptible (LS), weakly resistant (WR), and strongly resistant (SR). Multigenerational bioassays demonstrated a marked increase in resistance, with WR and SR populations exhibiting resistance ratios exceeding 30-fold following repeated phosphine exposure. Biochemical analyses revealed a significant decline in catalase activity (1.28-2.25-fold) in WR and SR populations, whereas peroxidase activity increased substantially, particularly in SR populations exposed to sublethal (3.25-fold) and high-dose (3.45-fold) treatments. Gene expression analysis normalized to RPS18, showed downregulation of catalase transcripts in WR and SR populations, alongside pronounced upregulation of peroxidase and superoxide dismutase (SOD) genes with increasing resistance. Multiple regression analysis revealed a strong association (R2 > 0.85) between resistance levels, antioxidant enzyme activities, and corresponding gene expression profiles. This study explores phosphine exposure, antioxidant defences, and resistance in T. castaneum, underscoring the need for continuous monitoring and adaptive management for sustainable resistance.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Biotechnology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s12033-026-01586-x","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

This study investigates the effects of sublethal and high-dose phosphine exposure on biocatalytic activity and gene expression of key antioxidants (catalase, peroxidase, and superoxide dismutase) in T. castaneum populations: susceptible (LS), weakly resistant (WR), and strongly resistant (SR). Multigenerational bioassays demonstrated a marked increase in resistance, with WR and SR populations exhibiting resistance ratios exceeding 30-fold following repeated phosphine exposure. Biochemical analyses revealed a significant decline in catalase activity (1.28-2.25-fold) in WR and SR populations, whereas peroxidase activity increased substantially, particularly in SR populations exposed to sublethal (3.25-fold) and high-dose (3.45-fold) treatments. Gene expression analysis normalized to RPS18, showed downregulation of catalase transcripts in WR and SR populations, alongside pronounced upregulation of peroxidase and superoxide dismutase (SOD) genes with increasing resistance. Multiple regression analysis revealed a strong association (R² > 0.85) between resistance levels, antioxidant enzyme activities, and corresponding gene expression profiles. This study explores phosphine exposure, antioxidant defences, and resistance in T. castaneum, underscoring the need for continuous monitoring and adaptive management for sustainable resistance.

查看原文本刊更多论文

磷化氢诱导的氧化应激下蓖麻的抗氧化恢复能力

本研究研究了亚致死和高剂量膦暴露对木栗易感（LS）、弱抗性（WR）和强抗性（SR）群体中过氧化氢酶、过氧化物酶和超氧化物歧化酶等关键抗氧化剂生物催化活性和基因表达的影响。多代生物测定显示抗性显著增加，重复接触磷化氢后，WR和SR种群的抗性比超过30倍。生化分析显示，在白带和白带种群中过氧化氢酶活性显著下降（1.28-2.25倍），而过氧化物酶活性显著增加，特别是在暴露于亚致死（3.25倍）和高剂量（3.45倍）处理的白带种群中。基因表达分析归一化至RPS18，结果显示，随着抗性的增加，WR和SR群体过氧化氢酶转录物下调，同时过氧化物酶和超氧化物歧化酶（SOD）基因明显上调。多元回归分析显示，抗性水平、抗氧化酶活性与相应基因表达谱之间存在较强的相关性（R2 > 0.85）。本研究探讨了蓖麻的磷化氢暴露、抗氧化防御和抗性，强调了持续监测和适应性管理的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Molecular Biotechnology 医学-生化与分子生物学

CiteScore

4.10

自引率

3.80%

发文量

165

审稿时长

6 months

期刊介绍： Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.