Blueberry leaf polyphenols suppress biofilm formation and restore oral microbial homeostasis for caries control.

IF 6.7 2区医学 Q2 MICROBIOLOGY

Journal of Oral Microbiology Pub Date : 2026-06-03 eCollection Date: 2026-01-01 DOI:10.1080/20002297.2026.2681284

Xuanlan Wu, Heng Xiao, Yanbing Chen, Chunqing Wu, Qiyuan Xie, Yu Gu, Xiaofei Qin

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引用次数: 0

Abstract

Background: Dental caries is a dysbiotic biofilm-induced disease driven by Streptococcus mutans (S. mutans). Blueberry leaf polyphenols (BLP) are plant-derived polyphenols exhibiting antimicrobial properties. However, their effects on S. mutans remain incompletely understood.

Methods: The composition of BLP was determined by ultrahigh-pressure liquid chromatography-triple quadrupole tandem mass spectrometer (UPLC-MS/MS). The minimum inhibitory concentration (MIC) and acid suppression activity were determined. In addition, the effect on biofilm was investigated by adhesion and aggregation assay, crystal violet assay, glycosyltransferases (GTF) activity, extracellular polysaccharides (EPS) quantification, scanning electron microscope (SEM) images and qRT-PCR. Furthermore, we carried out a rat caries model, and microbiome analyzes were performed by 16S rRNA sequencing.

Results: UPLC-MS/MS analysis identified a range of compounds in BLP, including quercetin and chlorogenic acid. The MIC of BLP was 500 μg/mL, and it inhibited the acid production of S. mutans. Importantly, BLP decreased the sucrose-dependent adhesion and its auto-aggregation, inhibited the activity of GTF, resulting in reduced EPS and down-regulated genes like gtfB, comA and gbpB. In addition, it inhibited dental caries in rats and ameliorated the microbiota dysbiosis associated with caries.

Conclusions: BLP combats caries by inhibiting S. mutans growth, biofilm formation and restoring the oral microbiota.

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蓝莓叶多酚抑制生物膜形成，恢复口腔微生物稳态，控制龋病。

背景：龋齿是一种由变形链球菌（S. mutans）引起的由生物膜引起的疾病。蓝莓叶多酚（BLP）是植物源性多酚，具有抗菌特性。然而，它们对变形链球菌的影响仍不完全清楚。方法：采用超高压液相色谱-三重四极杆串联质谱联用仪（UPLC-MS/MS）测定其成分。测定了最小抑酸浓度（MIC）和抑酸活性。此外，通过黏附和聚集实验、结晶紫实验、糖基转移酶（GTF）活性、胞外多糖（EPS）定量、扫描电镜（SEM）图像和qRT-PCR研究了其对生物膜的影响。此外，我们建立了大鼠龋齿模型，并通过16S rRNA测序进行微生物组分析。结果：UPLC-MS/MS分析鉴定出槲皮素、绿原酸等多种化合物。BLP的MIC为500 μg/mL，对变形链球菌产酸有抑制作用。重要的是，BLP降低了蔗糖依赖性粘附及其自动聚集，抑制了GTF的活性，导致EPS减少，gtfB、comA和gbpB等基因下调。此外，它还能抑制大鼠的龋齿，改善与龋齿相关的微生物群失调。结论：BLP通过抑制变形链球菌生长、生物膜形成和恢复口腔微生物群来对抗龋病。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Oral Microbiology MICROBIOLOGY-

CiteScore

8.00

自引率

4.40%

发文量

审稿时长

12 weeks

期刊介绍： As the first Open Access journal in its field, the Journal of Oral Microbiology aims to be an influential source of knowledge on the aetiological agents behind oral infectious diseases. The journal is an international forum for original research on all aspects of ''oral health''. Articles which seek to understand ''oral health'' through exploration of the pathogenesis, virulence, host-parasite interactions, and immunology of oral infections are of particular interest. However, the journal also welcomes work that addresses the global agenda of oral infectious diseases and articles that present new strategies for treatment and prevention or improvements to existing strategies. Topics: ''oral health'', microbiome, genomics, host-pathogen interactions, oral infections, aetiologic agents, pathogenesis, molecular microbiology systemic diseases, ecology/environmental microbiology, treatment, diagnostics, epidemiology, basic oral microbiology, and taxonomy/systematics. Article types: original articles, notes, review articles, mini-reviews and commentaries