Cytochrome P450 Monooxygenase Mediated Pyrethroid Resistance in the Urban Dengue Vector, Aedes aegypti (Diptera: Culicidae) from Northern Part of West Bengal, India
Prapti Das, Subhajit Das, Abhirup Saha, Abhinna Tamang, Dhiraj Saha
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引用次数: 0
Abstract
Purpose
Development of pyrethroid resistance in Aedes aegypti (Diptera: Culicidae) can lead to failure of ongoing vector control strategies and thereby, impede prevention of dengue transmission in urban regions of India. Therefore, its resistance status needs to be properly understood to prevent recurring dengue outbreaks in cities. The present study investigated cytochrome P450 monooxygenase (CYP)-mediated pyrethroid resistance in this vector species from dengue endemic northern part of West Bengal, India.
Methods
Larvae and pupae of wild Ae. aegypti populations were collected from urban regions of two districts (Siliguri town from Darjeeling and Raiganj from Uttar Dinajpur). Larval and adult bioassays were performed for two synthetic pyrethroids viz. deltamethrin and bifenthrin. Synergist assay with Piperonyl Butoxide (PBO), a CYP blocker, was conducted to understand the involvement of CYPs. Relative expression of four CYP9 family genes: CYP9J24, CYP9J26, CYP9J28 and CYP9J32 was also studied through Quantitative Reverse Transcription PCR (qRT-PCR) in larvae and adults.
Results
Larvae and adults of wild Ae. aegypti populations were found resistant for both pyrethroids. Pre-exposure to PBO restored susceptibility in both larvae and adults, indicates the involvement of CYPs in the observed resistance. Differential over-expression of the CYP9 family genes in larvae and adults strengthened association of CYPs with pyrethroid resistance of studied populations.
Conclusion
A control strategy targeting CYPs can be effective in management of Ae. aegypti in this region. Moreover, use of synergists like CYP blockers can help in restoration of susceptibility to pyrethroids in wild Ae. aegypti populations of northern part of West Bengal.
期刊介绍：
Acta Parasitologica is an international journal covering the latest advances in the subject.
Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews.
The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
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