Optical oxygen sensor based on luminescence quenching for dissolved oxygen (DO) detection: Metalloporphyrin embedded in fluorinated acrylate nanospheres and their biological application

IF 6.9 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports Pub Date : 2026-06-01 Epub Date: 2026-02-05 DOI:10.1016/j.snr.2026.100449

Honglin Zhang , Yang Sun , Wei Zhao , Guanjie Li , Ke Zhang , Jiupeng Zhao , Yao Li

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引用次数: 0

Abstract

Optical oxygen sensors based on the luminescence quenching mechanism were developed by encapsulating platinum octaethylporphyrin (PtOEP) into fluoropolymer nanospheres of Poly(methyl methacrylate-co-trifluoroethyl methacrylate) (Poly(MMA-co-TFEMA)) and Poly(methyl methacrylate-co-trifluoroethyl methacrylate-co‑butyl acrylate) (Poly(MMA-co-TFEMA-co-BA)) via miniemulsion polymerization for dissolved oxygen (DO) detection. The luminescence intensity of the oxygen-sensing nanospheres decreases significantly with increasing DO concentration, with maximum quenching ratios (I₀/I₁₀₀) estimated to be 6.19±0.15, and 8.92±0.26 for Poly(MMA-co-TFEMA) and Poly(MMA-co-TFEMA-co-BA) nanospheres, respectively. The response times to DO were 28 s and 24 s for Poly(MMA-co-TFEMA) and Poly(MMA-co-TFEMA-co-BA) nanospheres. After 1 h of continuous light irradiation, the decay ratios of luminescence intensity for both remained below 0.1%. Experimental results demonstrate that both nanosphere-based oxygen sensors exhibit high sensitivity and accuracy, fast response time, and excellent photostability. Finally, PtOEP@Poly(MMA-co-TFEMA-co-BA) nanospheres were used as a photosensitizer, and their photodynamic therapy (PDT) efficacy was evaluated in human A431 cells. In vitro studies indicate that cancer cells are effectively destroyed at a low dose of PtOEP@Poly(MMA-co-TFEMA-co-BA) nanospheres under light irradiation, suggesting the potential of these oxygen-sensing nanospheres for photodynamic therapy applications.

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基于发光猝灭的溶解氧（DO）检测光学氧传感器：含氟丙烯酸酯纳米球中金属卟啉的嵌入及其生物应用

采用微乳液聚合的方法，将八乙基卟啉铂（ptep）包封在聚（甲基丙烯酸甲酯-共三氟乙基甲基丙烯酸酯）（聚（MMA-co-TFEMA））和聚（甲基丙烯酸甲酯-共三氟乙基甲基丙烯酸酯-co-丁基丙烯酸酯）（聚（MMA-co-TFEMA-co- ba））的含氟聚合物纳米球中，开发了基于发光猝灭机理的光学氧传感器，用于溶解氧（DO）检测。随着DO浓度的增加，氧传感纳米球的发光强度显著降低，Poly（MMA-co-TFEMA）和Poly（MMA-co-TFEMA-co- ba）纳米球的最大猝灭比（I0/I100）分别为6.19±0.15和8.92±0.26。聚（MMA-co-TFEMA）和聚（MMA-co-TFEMA-co- ba）纳米球对DO的响应时间分别为28 s和24 s。连续光照射1 h后，两种材料的发光强度衰减率均保持在0.1%以下。实验结果表明，这两种纳米球氧传感器具有较高的灵敏度和精度、快速的响应时间和良好的光稳定性。最后，以PtOEP@Poly（MMA-co-TFEMA-co-BA）纳米微球作为光敏剂，评价其对人A431细胞的光动力治疗（PDT）效果。体外研究表明，低剂量的PtOEP@Poly（MMA-co-TFEMA-co-BA）纳米球在光照射下可以有效地破坏癌细胞，这表明这些氧传感纳米球在光动力治疗中的应用潜力。

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来源期刊

Sensors and Actuators Reports Multiple-

CiteScore

9.60

自引率

0.00%

发文量

审稿时长

49 days

期刊介绍： Sensors and Actuators Reports is a peer-reviewed open access journal launched out from the Sensors and Actuators journal family. Sensors and Actuators Reports is dedicated to publishing new and original works in the field of all type of sensors and actuators, including bio-, chemical-, physical-, and nano- sensors and actuators, which demonstrates significant progress beyond the current state of the art. The journal regularly publishes original research papers, reviews, and short communications. For research papers and short communications, the journal aims to publish the new and original work supported by experimental results and as such purely theoretical works are not accepted.