Comparative genomics reveals immunomodulatory and anti-obesity traits of Lactococcus lactis subsp. lactis

IF 4.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Applied Microbiology and Biotechnology Pub Date : 2026-06-06 Epub Date: 2026-08-11 DOI:10.1007/s00253-026-13892-0

Huijin Jeong, Hyukjoon Choi, Young-Seo Park

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Abstract

Lactococcus lactis subsp. lactis strains have been widely recognized for their probiotic potential, including their immunomodulatory and anti-obesity effects. This study aims to determine the genomic basis of the health-promoting properties of L. lactis subsp. lactis CAB701 and WiKim0124 using whole-genome sequencing and comparative genomic analysis. Taxonomic classification using average nucleotide identity and digital DNA-DNA hybridization confirms both strains as L. lactis subsp. lactis. Genome alignment reveals high synteny among CAB701, WiKim0124, and the reference strains, with minor structural variations. Functional annotation identifies genes associated with carbohydrate metabolism, biofilm formation, and immune modulation. Pan-genome analysis shows a substantial proportion of strain-specific genes, suggesting niche-specific adaptation. Notably, L. lactis subsp. lactis CAB701 and WiKim0124 harbor unique biosynthetic gene clusters for nisin Z and sactipeptides, which may contribute to their antimicrobial and immunomodulatory functions. Additionally, genes involved in short-chain fatty acid metabolism are identified, highlighting their potential role in metabolic regulation. These findings provide valuable insights into the functional genetics of L. lactis subsp. lactis and their applicability as probiotics for immune modulation and obesity management.

• Comparative genomics revealed strain-specific immune- and metabolism-linked genes

• CAB701 and WiKim0124 uniquely harbor nisin Z and sactipeptide biosynthetic clusters

• Genomic features support L. lactis application as functional probiotics

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比较基因组学揭示乳酸乳球菌亚种的免疫调节和抗肥胖特性。lactis。

乳酸乳球菌亚种乳酸杆菌菌株因其益生菌潜力而被广泛认可，包括其免疫调节和抗肥胖作用。本研究旨在确定乳酸菌亚种促进健康特性的基因组基础。lactis CAB701和WiKim0124使用全基因组测序和比较基因组分析。利用平均核苷酸鉴定和数字DNA-DNA杂交进行分类，证实两株菌株均为乳杆菌亚种。lactis。基因组比对显示，CAB701、WiKim0124和参考菌株具有较高的同源性，结构差异较小。功能注释确定了与碳水化合物代谢、生物膜形成和免疫调节相关的基因。泛基因组分析显示了相当大比例的菌株特异性基因，表明特定于小生境的适应。值得注意的是，乳酸菌亚种。lactis CAB701和WiKim0124含有独特的nisin Z和sactipeptide生物合成基因簇，这可能有助于它们的抗菌和免疫调节功能。此外，还发现了短链脂肪酸代谢相关基因，强调了它们在代谢调节中的潜在作用。这些发现为乳酸菌亚种的功能遗传学研究提供了有价值的见解。乳酸菌及其作为免疫调节和肥胖管理益生菌的适用性。•比较基因组学揭示了菌株特异性免疫和代谢相关基因•CAB701和WiKim0124独特地含有nisin Z和sactipeptide生物合成簇•基因组特征支持乳酸乳杆菌作为功能性益生菌的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Applied Microbiology and Biotechnology 工程技术-生物工程与应用微生物

CiteScore

10.00

自引率

4.00%

发文量

535

审稿时长

2 months

期刊介绍： Applied Microbiology and Biotechnology focusses on prokaryotic or eukaryotic cells, relevant enzymes and proteins; applied genetics and molecular biotechnology; genomics and proteomics; applied microbial and cell physiology; environmental biotechnology; process and products and more. The journal welcomes full-length papers and mini-reviews of new and emerging products, processes and technologies.