Characterization of nano α-TCP: Setting time, antibacterial activity and cytotoxicity on fibroblast cells

IF 2.7 Q2 MEDICINE, GENERAL & INTERNAL

Journal of Taibah University Medical Sciences Pub Date : 2026-06-01 Epub Date: 2026-06-05 DOI:10.1016/j.jtumed.2026.05.009

Nadya L. Riany Sp.KG , Selviana Wulansari Sp.KG (K) , Ade P. Dwisaptarini Sp.KG (K)., PhD , Moehamad O. Roeslan MKes., PhD., PBO. , Thalia C. Maharani SKG. , Faradilla J. Azzahra SKG.

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引用次数: 0

Abstract

Background

Nano α-Tricalcium Phosphate (nano α-TCP) is a potential pulp capping material due to its calcium/phosphate ion release, antibacterial properties, and biocompatibility.

Objectives

To evaluate the setting time, antibacterial activity against Streptococcus mutans, and cytotoxicity of nano α-TCP.

Methods

Setting time was measured using the Vicat test. Antibacterial activity was assessed by direct contact testing at concentrations of 100%, 50%, and 25%. Cytotoxicity on fibroblasts was evaluated using the CCK-8 assay after 24 and 72 hours.

Results

Nano α-TCP at 100% concentration showed the strongest antibacterial effect and the fastest setting time. All tested concentrations maintained high fibroblast viability, indicating low cytotoxicity.

Conclusion

Nano α-TCP effectively inhibits S. mutans, sets rapidly, and is biocompatible, supporting its potential as a pulp capping material.

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纳米α-TCP的表征：凝固时间、抗菌活性及对成纤维细胞的细胞毒性

纳米α-磷酸三钙（纳米α-TCP）由于其钙/磷酸盐离子释放、抗菌性能和生物相容性，是一种潜在的牙髓封盖材料。目的评价纳米α-TCP的凝固时间、对变形链球菌的抑菌活性及细胞毒性。方法采用Vicat法测定凝固时间。采用浓度为100%、50%和25%的直接接触试验评估抗菌活性。24小时和72小时后用CCK-8法评价成纤维细胞的细胞毒性。结果100%浓度的纳米α-TCP抗菌效果最强，凝固时间最快。所有测试浓度保持高成纤维细胞活力，表明低细胞毒性。结论纳米α-TCP能有效抑制变形链球菌，固化速度快，具有良好的生物相容性，具有作为牙髓封盖材料的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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