Transcriptomic Analysis Reveals the Effects of κ-Carrageenan Oligosaccharides on Tobacco Mosaic Virus Resistance

IF 1.2 4区农林科学 Q3 PLANT SCIENCES

Journal of Phytopathology Pub Date : 2026-05-28 DOI:10.1111/jph.70324

Guojie Sun, Xiaona Zhao, Haixin Hu, Pingping Zhang, Penyu Chen, Junyan Sun, Xiangyu Cong, Jiang Li

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引用次数: 0

Abstract

Seaweed oligosaccharides exhibit multiple biological activities and act as novel plant growth regulators. Our previous research indicates that carrageenan oligosaccharides (COs) can effectively control tobacco mosaic virus (TMV) disease. To reveal the COs defence mechanisms against tobacco viruses, a transcriptomic analysis was performed to explore the potential antiviral pathways of κ-COs. Compared with the control group, the treated groups exhibited substantial delays in disease onset, and their disease indices also markedly decreased. After TMV inoculation, compared with the control group, the defence enzyme activities, such as those of superoxide dismutase, β-1,3-glucanase, and peroxidase, in the treated groups significantly increased. The expression levels of key genes related to the resistance-associated pathways, such as Nicotiana rustica-derived Tobacco Mosaic Virus Resistance Gene (NrCN) and Mitogen-Activated Protein Kinase (MAPK), in the treated groups were higher than those in the untreated control group. In-depth gene sequencing revealed that a large number of genes and their corresponding metabolic pathways in treated groups showed extremely significant variations after inoculation with TMV. This study investigated the effective influence of COs on tobacco TMV resistance and examined the effects of carrageenan oligosaccharides on the metabolism of TMV-inoculated tobacco plants at the transcriptional level.

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转录组学分析揭示κ-卡拉胶寡糖对烟草花叶病毒抗性的影响

海藻寡糖具有多种生物活性，是一种新型的植物生长调节剂。前期研究表明，卡拉胶寡糖能有效防治烟草花叶病毒（TMV）病。为了揭示COs对烟草病毒的防御机制，我们通过转录组学分析来探索κ-COs潜在的抗病毒途径。与对照组相比，治疗组的发病时间明显延迟，疾病指标也明显降低。接种TMV后，与对照组相比，各处理组的超氧化物歧化酶、β-1,3-葡聚糖酶、过氧化物酶等防御酶活性均显著升高。处理组烟草花叶病毒抗性基因（NrCN）和丝裂原活化蛋白激酶（MAPK）等与抗性相关途径相关的关键基因的表达水平高于未处理组。深入的基因测序显示，接种TMV后，处理组大量基因及其相应的代谢途径出现了极显著的变异。本研究研究了COs对烟草TMV抗性的有效影响，并在转录水平上考察了卡拉胶寡糖对接种TMV的烟草植株代谢的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Phytopathology 生物-植物科学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays. Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes. Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.