Lucia Franco-Dilone, Oitak Allen Wong, Omar F Laterza, Xuemei Zhao
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引用次数: 0
Abstract
To assess circulating CXCL9 as an exploratory biomarker in clinical studies, an electrochemiluminescence CXCL9 assay was validated in human serum to define assay conditions that can remove matrix effect, improve precision and accuracy, as well as establish sensitivity and reportable range. The validated assay demonstrates ultra-sensitivity with a lower limit of quantification of 1.64 pg/mL in human serum. This assay exhibits optimal precision and accuracy. In addition, biological variability and CXCL9 stability in human serum were also assessed. The validated assay is an exploratory clinical biomarker assay that enables assessment of CXCL9 as an exploratory pharmacodynamic or predictive marker that correlates with clinical response in clinical studies.
期刊介绍：
BioTechniques is a peer-reviewed, open-access journal dedicated to publishing original laboratory methods, related technical and software tools, and methods-oriented review articles that are of broad interest to professional life scientists, as well as to scientists from other disciplines (e.g., chemistry, physics, computer science, plant and agricultural science and climate science) interested in life science applications for their technologies.
Since 1983, BioTechniques has been a leading peer-reviewed journal for methods-related research. The journal considers:
Reports describing innovative new methods, platforms and software, substantive modifications to existing methods, or innovative applications of existing methods, techniques & tools to new models or scientific questions
Descriptions of technical tools that facilitate the design or performance of experiments or data analysis, such as software and simple laboratory devices
Surveys of technical approaches related to broad fields of research
Reviews discussing advancements in techniques and methods related to broad fields of research
Letters to the Editor and Expert Opinions highlighting interesting observations or cautionary tales concerning experimental design, methodology or analysis.
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