Measuring Dynamic Inflammation Regulation in Low-Resource Settings: Results From a Sample of Adults in the Dominican Republic

IF 1.9 4区医学 Q1 ANTHROPOLOGY

American Journal of Human Biology Pub Date : 2026-06-01 DOI:10.1002/ajhb.70286

Keegan C. Krause, Sayira Paola Mueses Jiménez, Franceska Duperval, Farah St. Juste, Aaron A. Miller, Thomas W. McDade

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引用次数: 0

Abstract

Introduction

Inflammatory signaling mediates several homeostatic mechanisms that shape health, and quantifying inflammation regulation patterns using ex vivo cell culture systems—response to activation and sensitivity to inhibition—improves upon baseline measures of chronic inflammation. The purpose of this report is to demonstrate the feasibility of implementing an ex vivo cell culture system in a low-resource setting in the Dominican Republic.

Methods

This study implements an ex vivo cell culture system to measure stimulated inflammation and glucocorticoid (GC) sensitivity using finger stick capillary blood collected from 202 adults in the Dominican Republic (age: 18–61 years).

Results

Median cytokine responses (pg/mL) to incubation with lipopolysaccharide (LPS) were robust for IL6 (58.0), IL1β (21.3), and TNFα (23.1). Median cytokine responses to incubation with LPS and GC—capturing sensitivity to inhibition—were attenuated for IL6 (20.2), IL1β (8.4), and TNFα (7.0).

Conclusion

Minimally invasive cell culture protocols offer novel research opportunities for measuring inflammation regulation and health in low-resource settings across diverse eco-social milieus.

Abstract Image

查看原文本刊更多论文

在低资源环境下测量动态炎症调节：来自多米尼加共和国成人样本的结果。

炎症信号介导了几种塑造健康的稳态机制，使用离体细胞培养系统量化炎症调节模式-对激活的反应和对抑制的敏感性-改善了慢性炎症的基线测量。本报告的目的是证明在多米尼加共和国资源匮乏的环境中实施离体细胞培养系统的可行性。方法：本研究实施了一个体外细胞培养系统，使用收集自多米尼加共和国202名成年人（年龄：18-61岁）的手指棒毛细血管血液来测量刺激炎症和糖皮质激素（GC）敏感性。结果：与脂多糖（LPS）孵育的中位数细胞因子（pg/mL）对IL6 (58.0), IL1β(21.3)和TNFα(23.1)的反应强劲。与LPS和gc孵育的中位数细胞因子反应-捕获对抑制的敏感性- IL6 (20.2), IL1β(8.4)和TNFα(7.0)减弱。结论：微创细胞培养方案为测量不同生态社会环境下低资源环境下的炎症调节和健康状况提供了新的研究机会。

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来源期刊

American Journal of Human Biology 生物-生物学

CiteScore

4.80

自引率

13.80%

发文量

124

审稿时长

4-8 weeks

期刊介绍： The American Journal of Human Biology is the Official Journal of the Human Biology Association. The American Journal of Human Biology is a bimonthly, peer-reviewed, internationally circulated journal that publishes reports of original research, theoretical articles and timely reviews, and brief communications in the interdisciplinary field of human biology. As the official journal of the Human Biology Association, the Journal also publishes abstracts of research presented at its annual scientific meeting and book reviews relevant to the field. The Journal seeks scholarly manuscripts that address all aspects of human biology, health, and disease, particularly those that stress comparative, developmental, ecological, or evolutionary perspectives. The transdisciplinary areas covered in the Journal include, but are not limited to, epidemiology, genetic variation, population biology and demography, physiology, anatomy, nutrition, growth and aging, physical performance, physical activity and fitness, ecology, and evolution, along with their interactions. The Journal publishes basic, applied, and methodologically oriented research from all areas, including measurement, analytical techniques and strategies, and computer applications in human biology. Like many other biologically oriented disciplines, the field of human biology has undergone considerable growth and diversification in recent years, and the expansion of the aims and scope of the Journal is a reflection of this growth and membership diversification. The Journal is committed to prompt review, and priority publication is given to manuscripts with novel or timely findings, and to manuscripts of unusual interest.