Pyramiding nn16 and rin1 alleles to balance plant height and node number at high latitudes.

IF 3.6 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2026-05-29 eCollection Date: 2026-06-01 DOI:10.1007/s11032-026-01682-5

Jialin Zhang, Yulin Wang, Jiemin Chen, Sijia Lu, Baohui Liu, Tong Su, Shichen Li

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引用次数: 0

Abstract

Breeding dwarf or semi-dwarf soybean varieties is an effective strategy to enhance lodging resistance and thereby increase yield potential. However, soybean plant height is co-determined by the number of nodes and internode length. Reducing plant height often leads to a decrease in node number, which subsequently reduces pod number and ultimately results in lower yield. Thus, balancing node number and internode length is of great significance for increasing soybean yield. Here, we identified a node number-regulating locus, NUMBER of NODES 16 (NN16), encoded by FLOWERING LOCUS T (FT5a). Variations in the promoter and 3' untranslated region (3' UTR) of nn16 downregulate its expression and increase node number. In addition, the reduced internode 1 (rin1) gene that we previously reported shortens internode length but slightly reduces node number. To increase node number while shortening internode length, we constructed near-isogenic lines (NILs) carrying the genotypes RIN1 ^HH43 /NN16, rin1/nn16, RIN1 ^HH43 /nn16, and rin1/NN16. Among these NILs, the rin1/nn16 genotype showed optimal plant height and the highest seed weight per plant. Collectively, our findings provide theoretical support and breeding resources for cultivating soybean cultivars with shortened internodes and increased node number, thereby further improving seed weight per plant.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-026-01682-5.

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利用nn16和rin1等位基因在高纬度地区平衡株高和节数。

选育矮秆或半矮秆大豆品种是提高大豆抗倒伏能力从而提高产量潜力的有效策略。而大豆株高是由节数和节间长共同决定的。降低株高往往导致节数减少，进而导致荚果数减少，最终导致产量降低。因此，平衡节点数和节间长度对提高大豆产量具有重要意义。在这里，我们发现了一个由开花位点T （FT5a）编码的节点数量调节位点NUMBER of NODES 16 （NN16）。nn16启动子和3′非翻译区（3′UTR）的变化下调了其表达，增加了节点数。此外，我们之前报道的节间1 （rin1）基因缩短了节间长度，但略微减少了节点数。为了增加结数，缩短节间长度，我们构建了携带RIN1 HH43 /NN16、RIN1 /NN16、RIN1 HH43 /NN16和RIN1 /NN16基因型的近等基因系（NILs）。其中，rin1/nn16基因型株高最佳，单株粒重最高。本研究结果为培育节间缩短、节数增加的大豆品种，进一步提高单株种子重提供了理论支持和育种资源。补充资料：在线版本包含补充资料，下载地址：10.1007/s11032-026-01682-5。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.