Elucidating therapeutic mechanisms of naringin and phloridzin on ulcerative colitis mice by metabolomics-based comparative analysis

IF 5.8 3区化学 Q1 CHEMISTRY, MEDICINAL

Natural Products and Bioprospecting Pub Date : 2026-06-01 DOI:10.1007/s13659-026-00620-4

Jing Yang, Chongxin Kang, Chulei Xiao, Yusi Zhang, Yihong Mai, Yinghong Huang, Yi Zhan, Hetong Liu, Xian Wang

{"title":"Elucidating therapeutic mechanisms of naringin and phloridzin on ulcerative colitis mice by metabolomics-based comparative analysis","authors":"Jing Yang, Chongxin Kang, Chulei Xiao, Yusi Zhang, Yihong Mai, Yinghong Huang, Yi Zhan, Hetong Liu, Xian Wang","doi":"10.1007/s13659-026-00620-4","DOIUrl":null,"url":null,"abstract":"<div><p>Naringin and phloridzin are structurally similar flavonoids with anti-inflammatory, antioxidant, and antimicrobial properties, showing considerable potential as natural therapeutic agents. This study demonstrated that naringin and phloridzin exert anti-inflammatory effects and preserve normal intestinal function in UC mice. This protective effect is mediated by inhibition of the NF-κB signalling pathway. To investigate metabolic differences, we employed UPLC-MS/MS-based widely targeted metabolomics alongside targeted analyses of tryptophan (Trp) and short-chain fatty acids (SCFAs). Comparative analysis of metabolic profiles, differential metabolites, and associated pathways revealed regulatory effects on Trp and SCFAs. However, naringin demonstrated superior regulation of Trp metabolism and SCFA levels compared to phloridzin, resulting in more pronounced improvement of gut homeostasis. These findings elucidate the metabolic mechanisms underlying both flavonoid treatments and underscore their potential as complementary therapies for UC.</p><h3>Graphical abstract</h3><p>Naringin and phloridzin exert protective effects against DSS-induced ulcerative colitis by modulating tryptophan metabolism and suppressing inflammation through inhibition of the NF-κB signaling pathway</p>\n<div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":718,"journal":{"name":"Natural Products and Bioprospecting","volume":"16 1","pages":""},"PeriodicalIF":5.8000,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13659-026-00620-4.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Natural Products and Bioprospecting","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s13659-026-00620-4","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}

引用次数: 0

Abstract

Naringin and phloridzin are structurally similar flavonoids with anti-inflammatory, antioxidant, and antimicrobial properties, showing considerable potential as natural therapeutic agents. This study demonstrated that naringin and phloridzin exert anti-inflammatory effects and preserve normal intestinal function in UC mice. This protective effect is mediated by inhibition of the NF-κB signalling pathway. To investigate metabolic differences, we employed UPLC-MS/MS-based widely targeted metabolomics alongside targeted analyses of tryptophan (Trp) and short-chain fatty acids (SCFAs). Comparative analysis of metabolic profiles, differential metabolites, and associated pathways revealed regulatory effects on Trp and SCFAs. However, naringin demonstrated superior regulation of Trp metabolism and SCFA levels compared to phloridzin, resulting in more pronounced improvement of gut homeostasis. These findings elucidate the metabolic mechanisms underlying both flavonoid treatments and underscore their potential as complementary therapies for UC.

Graphical abstract

Naringin and phloridzin exert protective effects against DSS-induced ulcerative colitis by modulating tryptophan metabolism and suppressing inflammation through inhibition of the NF-κB signaling pathway

The alternative text for this image may have been generated using AI.

查看原文本刊更多论文

通过代谢组学的比较分析阐明柚皮苷和连根苷对溃疡性结肠炎小鼠的治疗机制。

柚皮苷和连根苷是结构相似的类黄酮，具有抗炎、抗氧化和抗菌特性，作为天然治疗剂具有很大的潜力。本研究表明，柚皮苷和连根苷具有抗炎作用，并能维持UC小鼠正常肠道功能。这种保护作用是通过抑制NF-κB信号通路介导的。为了研究代谢差异，我们采用了基于UPLC-MS/ ms的广泛靶向代谢组学以及色氨酸（Trp）和短链脂肪酸（SCFAs）的靶向分析。代谢谱、差异代谢物和相关途径的比较分析揭示了对色氨酸和SCFAs的调节作用。然而，与根内苷相比，柚皮苷对色氨酸代谢和SCFA水平的调节能力更强，从而更明显地改善肠道稳态。这些发现阐明了黄酮类治疗的代谢机制，并强调了它们作为UC补充治疗的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Natural Products and Bioprospecting CHEMISTRY, MEDICINAL-

CiteScore

8.30

自引率

2.10%

发文量

审稿时长

13 weeks

期刊介绍： Natural Products and Bioprospecting serves as an international forum for essential research on natural products and focuses on, but is not limited to, the following aspects: Natural products: isolation and structure elucidation Natural products: synthesis Biological evaluation of biologically active natural products Bioorganic and medicinal chemistry Biosynthesis and microbiological transformation Fermentation and plant tissue cultures Bioprospecting of natural products from natural resources All research articles published in this journal have undergone rigorous peer review. In addition to original research articles, Natural Products and Bioprospecting publishes reviews and short communications, aiming to rapidly disseminate the research results of timely interest, and comprehensive reviews of emerging topics in all the areas of natural products. It is also an open access journal, which provides free access to its articles to anyone, anywhere.