Bacterial Diversity and Functional Dynamics in the Soybean (Glycine max L.) Rhizosphere Under Different Organic Fertilisation.

IF 3.1 4区生物学 Q2 ENVIRONMENTAL SCIENCES

Environmental Microbiology Reports Pub Date : 2026-06-01 DOI:10.1111/1758-2229.70370

Ijeoma Emelda Osuji, Akinlolu Olalekan Akanmu, Olubukola Oluranti Babalola

{"title":"Bacterial Diversity and Functional Dynamics in the Soybean (Glycine max L.) Rhizosphere Under Different Organic Fertilisation.","authors":"Ijeoma Emelda Osuji, Akinlolu Olalekan Akanmu, Olubukola Oluranti Babalola","doi":"10.1111/1758-2229.70370","DOIUrl":null,"url":null,"abstract":"<p><p>Soybean (Glycine max L.) is a globally important legume for oil and protein production, yet its responses to specific organic fertilisation practices remain insufficiently understood. Organic amendments such as cattle dung and poultry manure are sustainable alternatives to inorganic fertilisers, but their effects on the soybean rhizosphere microbiome remain poorly characterised. This study investigated microbial community structure and functional diversity under poultry manure and cattle dung treatments. Rhizospheric soils were collected from treated plots, untreated controls and bulk soil and then subjected to physicochemical analysis. DNA extracted from samples was analysed using 16S rRNA gene sequencing on the Illumina NovaSeq 6000 platform, with data processed in QIIME 2 (v2019.1). Poultry manure increased available phosphorus (28%) and calcium (19%), while cattle dung enhanced potassium (22%) and magnesium (17%). Microbial community composition shifted significantly, with poultry manure promoting copiotrophic taxa such as Burkholderia and Cupriavidus and cattle dung enriching decomposers including Paenibacillus and Treponema. Alpha diversity was highest in poultry manure (Shannon index 6.2) and bulk soil (6.0) and lowest in cattle dung (5.1). Functional predictions indicated retention of core metabolic pathways, suggesting functional redundancy. Overall, organic fertilisation reshapes microbial communities while maintaining essential functions, supporting sustainable soybean cultivation.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"18 3","pages":"e70370"},"PeriodicalIF":3.1000,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13239308/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Microbiology Reports","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/1758-2229.70370","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Soybean (Glycine max L.) is a globally important legume for oil and protein production, yet its responses to specific organic fertilisation practices remain insufficiently understood. Organic amendments such as cattle dung and poultry manure are sustainable alternatives to inorganic fertilisers, but their effects on the soybean rhizosphere microbiome remain poorly characterised. This study investigated microbial community structure and functional diversity under poultry manure and cattle dung treatments. Rhizospheric soils were collected from treated plots, untreated controls and bulk soil and then subjected to physicochemical analysis. DNA extracted from samples was analysed using 16S rRNA gene sequencing on the Illumina NovaSeq 6000 platform, with data processed in QIIME 2 (v2019.1). Poultry manure increased available phosphorus (28%) and calcium (19%), while cattle dung enhanced potassium (22%) and magnesium (17%). Microbial community composition shifted significantly, with poultry manure promoting copiotrophic taxa such as Burkholderia and Cupriavidus and cattle dung enriching decomposers including Paenibacillus and Treponema. Alpha diversity was highest in poultry manure (Shannon index 6.2) and bulk soil (6.0) and lowest in cattle dung (5.1). Functional predictions indicated retention of core metabolic pathways, suggesting functional redundancy. Overall, organic fertilisation reshapes microbial communities while maintaining essential functions, supporting sustainable soybean cultivation.

查看原文本刊更多论文

大豆（Glycine max L.）细菌多样性及其功能动态不同有机肥处理下的根际土壤。

大豆（Glycine max L.）是全球重要的石油和蛋白质生产豆类，但其对特定有机肥做法的反应仍未充分了解。牛粪和禽粪等有机改良剂是无机肥料的可持续替代品，但它们对大豆根际微生物群的影响仍不清楚。研究了禽粪和牛粪处理下微生物群落结构和功能多样性。从处理过的地块、未处理的对照和散装土壤中收集根际土壤，然后进行物理化学分析。样品中提取的DNA在Illumina NovaSeq 6000平台上使用16S rRNA基因测序进行分析，数据在QIIME 2 （v2019.1）中处理。禽粪提高了有效磷（28%）和钙（19%），牛粪提高了钾（22%）和镁（17%）。微生物群落组成发生显著变化，禽粪促进了伯克霍尔德菌和铜菌等共养类群的生长，牛粪促进了类芽孢杆菌和密螺旋体等分解体的生长。α多样性在禽粪（Shannon指数6.2）和散装土（6.0）中最高，在牛粪（5.1）中最低。功能预测显示核心代谢途径保留，提示功能冗余。总体而言，有机肥重塑了微生物群落，同时保持了基本功能，支持了大豆的可持续种植。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Environmental Microbiology Reports ENVIRONMENTAL SCIENCES-MICROBIOLOGY

CiteScore

6.00

自引率

3.00%

发文量

审稿时长

3.0 months

期刊介绍： The journal is identical in scope to Environmental Microbiology, shares the same editorial team and submission site, and will apply the same high level acceptance criteria. The two journals will be mutually supportive and evolve side-by-side. Environmental Microbiology Reports provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens.