Antibacterial and osteogenic effect of EGCG/HAP/AgNPs composite coating on titanium surface

IF 4.7 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine Pub Date : 2026-05-30 Epub Date: 2026-08-03 DOI:10.1007/s10856-026-07083-6

Jiayi Liao, Zhihua Li

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引用次数: 0

Abstract

Titanium implants are widely used in dental applications; however, their clinical application is still limited by challenges such as bacterial infection and poor osseointegration. In this study, a composite coating consisting of epigallocatechin-3-gallate (EGCG), hydroxyapatite (HAP), and silver nanoparticles (AgNPs) was fabricated on the titanium surface to improve its antibacterial activity and osteogenic potential. The coating was prepared via alkali-thermal treatment, followed by EGCG adhesion and subsequent deposition of HAP and AgNPs. Surface characterization confirmed successful construction of the coating with improved hydrophilicity. The EGCG/HAP/AgNPs coating showed gradual changes in surface morphology and elemental composition after PBS immersion, suggesting partial coating degradation while retaining detectable residual Ag, Ca, and P on the titanium surface. The coating also exhibited effective antibacterial activity against Staphylococcus aureus and significantly promoted osteogenic differentiation of MC3T3-E1 cells, as evidenced by enhanced alkaline phosphatase activity and mineralized nodule formation. These results demonstrate that the EGCG/HAP/AgNPs composite coating possesses good antibacterial capacity, osteogenic activity, and biocompatibility, showing great potential for improving the clinical performance of titanium implants.

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EGCG/HAP/AgNPs复合涂层在钛表面的抗菌和成骨作用。

钛种植体在牙科应用中应用广泛；然而，它们的临床应用仍然受到细菌感染和骨整合不良等挑战的限制。本研究在钛表面制备了一种由表没食子儿茶素-3-没食子酸酯（EGCG）、羟基磷灰石（HAP）和银纳米粒子（AgNPs）组成的复合涂层，以提高其抗菌活性和成骨潜能。通过碱热处理、EGCG黏附、HAP和AgNPs沉积制备涂层。表面表征证实了涂层的成功构建，并改善了亲水性。PBS浸泡后，EGCG/HAP/AgNPs涂层表面形貌和元素组成逐渐发生变化，表明涂层部分降解，但在钛表面保留了可检测的Ag、Ca和P残留。该涂层还表现出对金黄色葡萄球菌的有效抗菌活性，并显著促进MC3T3-E1细胞的成骨分化，这可以通过增强碱性磷酸酶活性和矿化结节形成来证明。上述结果表明，EGCG/HAP/AgNPs复合涂层具有良好的抗菌能力、成骨活性和生物相容性，在提高钛种植体临床性能方面具有很大的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Materials Science: Materials in Medicine 工程技术-材料科学：生物材料

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

3.5 months

期刊介绍： The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.