Reference intervals for complete blood count parameters in the Longitudinal Study of Adult Health (ELSA-Brasil): a cross-sectional analysis.

IF 1.2 4区医学 Q2 MEDICINE, GENERAL & INTERNAL

Sao Paulo Medical Journal Pub Date : 2026-05-22 eCollection Date: 2026-01-01 DOI:10.1590/1516-3180.2025.3017.09122025

Nívea Aparecida Almeida, Sandhi Maria Barreto, Letícia Gonçalves Resende Ferreira, Chams Bicalho Maluf, Pedro Guatimosim Vidigal, Roberta Carvalho Figueiredo, Danyelle Romana Alves Rios

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引用次数: 0

Abstract

Background: Complete blood count (CBC) is the most frequently requested laboratory test worldwide, providing essential clinical information. In Brazil, many laboratories still use reference intervals (RIs) that are inadequately defined or not representative of the local population. Establishing population-specific RIs is crucial for accurate interpretation, diagnosis, and clinical decision making.

Objective: To establish RIs for CBC parameters in a sample of Brazilian adults.

Methods: This cross-sectional study included 2,417 healthy individuals who participated in the baseline (2008-2010) of the Longitudinal Study of Adult Health (ELSA-Brasil). Venous blood collection and storage were performed according to the procedures established by the Clinical and Laboratory Standards Institute (CLSI) in 2018. CBC was performed in laboratories with laboratory proficiency. The RIs were calculated using the nonparametric method proposed by the CLSI.

Results: The RIs were stratified by sex only for the following parameters: Red blood cells (RBC; × 106/mm3): Male (4.4-5.6), Female (3.9-5.1); Hemoglobin (g/dL): Male (13.2-16.7), Female (11.8-14.9); Hematocrit (%): Male (39-49), Female (35.3-44.2); Mean corpuscular hemoglobin concentration (MCHC; g/dL): Male (32.3-35.7), Female (32-35.1); Platelets (× 103/mm3): Male (146-319), Female: (170-352). The other parameters were as follows: mean corpuscular volume (MCV) (fL): (79.9-96); mean corpuscular hemoglobin (MCH) (pg): (26.4-32.5); White blood cells (WBC; /mm3): (3,700-8,610); Neutrophils (/mm 3): (1,666-5,705); Eosinophils (/mm3): (23.8-530); Basophils (/mm3): (0-112); Lymphocytes (/mm3): (1,121-2,824); Monocytes (/mm3): (240-751.7).

Conclusion: Our results agree with those of other studies that have proposed RIs for CBC parameters. The differences found in MCV, neutrophil and WBC parameters (total population), and MCHC (both sexes) may be due to differences in the study populations, sample sizes, and pre-analytical and analytical study variables.

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成人健康纵向研究（ELSA-Brasil）中全血细胞计数参数的参考区间：横断面分析。

背景：全血细胞计数（CBC）是世界范围内最常被要求的实验室检测，提供重要的临床信息。在巴西，许多实验室仍然使用不充分定义或不能代表当地人口的参考区间（RIs）。建立人群特异性RIs对于准确解释、诊断和临床决策至关重要。目的：建立巴西成年人CBC参数的RIs。方法：这项横断面研究包括2417名健康个体，他们参加了成人健康纵向研究（ELSA-Brasil）的基线（2008-2010）。静脉血采集和储存按照临床与实验室标准协会（CLSI） 2018年制定的程序进行。全血细胞计数在实验室熟练进行。RIs采用CLSI提出的非参数方法计算。结果：RIs仅按以下参数按性别分层：红细胞（RBC； × 106/mm3）：男性（4.4-5.6），女性（3.9-5.1）；血红蛋白（g/dL）：男性（13.2-16.7），女性（11.8-14.9）；红细胞压积（%）：男性（39-49），女性（35.3-44.2）；平均红细胞血红蛋白浓度（MCHC; g/dL）：男性（32.3-35.7），女性（32-35.1）；血小板（× 103/mm3）：男性（146-319），女性（170-352）。其他参数：平均红细胞体积（MCV）（fL）：（79.9 ~ 96）；平均红细胞血红蛋白（MCH）（pg）：（26.4-32.5）；白细胞（WBC; /mm3）：（3,700-8,610）；中性粒细胞（/mm 3）：（1,666-5,705）；嗜酸性粒细胞（/mm3）：（23.8-530）；嗜碱性粒细胞（/mm3）：（0-112）；淋巴细胞（/mm3）：（1,121 ~ 2,824）；单核细胞（/mm3）：（240-751.7）。结论：我们的结果与其他提出CBC参数RIs的研究结果一致。MCV、中性粒细胞和白细胞参数（总体）以及MCHC（两性）的差异可能是由于研究人群、样本量以及分析前和分析研究变量的差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Sao Paulo Medical Journal 医学-医学：内科

CiteScore

2.20

自引率

7.10%

发文量

210

审稿时长

6-12 weeks

期刊介绍： Published bimonthly by the Associação Paulista de Medicina, the journal accepts articles in the fields of clinical health science (internal medicine, gynecology and obstetrics, mental health, surgery, pediatrics and public health). Articles will be accepted in the form of original articles (clinical trials, cohort, case-control, prevalence, incidence, accuracy and cost-effectiveness studies and systematic reviews with or without meta-analysis), narrative reviews of the literature, case reports, short communications and letters to the editor. Papers with a commercial objective will not be accepted.