Evaluation of the Osteogenic Potential of Periodontal Ligament and Tooth-Derived Substrates Effect on Osteogenesis: An In Vitro Pilot Study.

IF 0.7 Q4 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Maxillofacial & Oral Surgery Pub Date : 2026-06-01 Epub Date: 2024-08-21 DOI:10.1007/s12663-024-02311-4

Tiago Pinto Carvalho, Thiago Resck, Davi Kirsch, Marcelo Sperandio, Marcelo Henrique Napimoga, Júlio César Joly, Gustavo Vicentis Oliveira Fernandes, Daiane Cristina Peruzzo

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引用次数: 0

Abstract

Objective: The goal of this in vitro pilot study was to compare the osteogenic potential of different ground human tooth preparations.

Methods: Twelve maxillary third molars were included and divided into four groups: FT (full tooth), containing all dental tissues (enamel, dentin, pulp, cementum, and periodontal ligament); WE, without enamel; WPL, without periodontal ligament; and WEPL, without enamel and periodontal ligament. The teeth were ground to macroscopically homogeneous particles. The new bone formation was the primary variable evaluated. Pre-osteoblastic cells were incubated with protein extracted from this substrate to verify their osteoinductive potential. Cell proliferation, viability assays, mRNA expression of type I Collagen (COL-I), RUNX-2, BMP-2, and mineral nodules were assessed to achieve the main outcome. Data were analyzed using two-way ANOVA and Tukey tests, considering alpha = 5%.

Results: The FT group had the lowest proliferation cell rates, whereas WEPL had the highest rates (p < 0.05). Moreover, there was an increased gene expression of all genes studied in the WEPL group and a greater formation of phosphate nodules (p < 0.05).

Conclusion: The dental substrate without enamel and periodontal ligament (WEPL) showed better and improved results than the other groups, revealing promising osteogenic potential for use as a biomaterial for grafting.

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牙周韧带成骨潜能及牙源性基质成骨作用的体外初步研究。

目的：比较不同人牙基质制剂的成骨潜能。方法：将12颗上颌第三磨牙分为4组：FT（全牙）组，包括牙釉质、牙本质、牙髓、牙骨质和牙周韧带等全部牙组织；我们，没有珐琅；WPL，无牙周韧带；无牙釉质和牙周韧带的WEPL。牙齿被磨成宏观上均匀的颗粒。新骨形成是评估的主要变量。成骨前细胞与从该底物中提取的蛋白质孵育，以验证其成骨诱导潜能。通过细胞增殖、活力测定、I型胶原蛋白（COL-I）、RUNX-2、BMP-2和矿物结节的mRNA表达进行评估，以获得主要结果。数据分析采用双因素方差分析和Tukey检验，考虑alpha = 5%。结果：FT组的细胞增殖率最低，而WEPL组的细胞增殖率最高（p p）。结论：无牙釉质和牙周韧带的牙基质（WEPL）较其他组表现出更好和改善的效果，显示出作为生物材料移植的良好潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Maxillofacial & Oral Surgery DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

1.90

自引率

0.00%

发文量

138

期刊介绍： This journal offers comprehensive coverage of new techniques, important developments and innovative ideas in Oral and Maxillofacial Surgery. Practice-applicable articles help develop the methods used to handle dentoalveolar surgery, facial injuries and deformities, TMJ disorders, oral cancer, jaw reconstruction, anesthesia and analgesia. The journal also includes specifics on new instruments, diagnostic equipment’s and modern therapeutic drugs and devices. Journal of Oral and Maxillofacial Surgery is recommended for first or priority subscription by the Dental Section of the Medical Library Association. Specific topics covered recently have included: ? distraction osteogenesis ? synthetic bone substitutes ? fibroblast growth factors ? fetal wound healing ? skull base surgery ? computer-assisted surgery ? vascularized bone grafts Benefits to authorsWe also provide many author benefits, such as free PDFs, a liberal copyright policy, special discounts on Elsevier publications and much more. Please click here for more information on our author services.