Human P(3)450: cDNA and complete protein sequence, repetitive Alu sequences in the 3' nontranslated region, and localization of gene to chromosome 15.

Journal of Experimental Pathology Pub Date : 1987-01-01
A K Jaiswal, D W Nebert, O W McBride, F J Gonzalez
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Abstract

P(1)450 and P(3)450 are the two members of the dioxin-inducible P450 gene family, one of at least eight families in the entire P450 gene superfamily. The human P(1)450 gene has been previously sequenced. In this report the human liver P(3)450 protein is shown to migrate as a 54-kDa band on NaDodSO4-polyacrylamide gel electrophoresis. The human P(3)450 cDNA (3,064 bp) and complete P(3)450 protein (515 residues; Mr = 58,294) were determined. The human P(3)450 mRNA (3.3 kb) has an unusually long 3' nontranslated region (1,509 bp) primarily due to the inclusion of four copies of Alu repetitive sequences. Comparison of human P(3)450 cDNA with human P(1)450 cDNA and mouse (or rat) P(3)450 cDNA with mouse (or rat) P(1)450 cDNA suggests that an intra-exonic gene conversion event (in the region between 100 and 400 nucleotides from the 5' end of the translated region) most likely occurred between 20 and 80 million years ago. Analysis of 32 human X mouse and 23 human X hamster somatic cell hybrids confirm unequivocally that both P(3)450 and P(1)450 reside on human chromosome 15.

人类P(3)450: cDNA和完整的蛋白序列,3'非翻译区重复的Alu序列,基因定位到15号染色体。
P(1)450和P(3)450是可诱导二恶英的P450基因家族的两个成员,是整个P450基因超家族中至少8个家族中的一个。人类P(1)450基因此前已被测序。在本报告中,人类肝脏P(3)450蛋白在nadodso4 -聚丙烯酰胺凝胶电泳上以54 kda的条带迁移。人P(3)450 cDNA (3064 bp)和完整的P(3)450蛋白(515个残基);Mr = 58,294)。人类P(3)450 mRNA (3.3 kb)具有一个异常长的3'非翻译区(1,509 bp),这主要是由于包含四个拷贝的Alu重复序列。人类P(3)450 cDNA与人类P(1)450 cDNA以及小鼠(或大鼠)P(3)450 cDNA与小鼠(或大鼠)P(1)450 cDNA的比较表明,外显子内基因转换事件(在翻译区域5'端100至400个核苷酸之间的区域)很可能发生在2000万至8000万年前。对32例人类X小鼠和23例人类X仓鼠体细胞杂交的分析明确证实,P(3)450和P(1)450都存在于人类第15染色体上。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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