Establishment and Characterization of Canine Malignant Mesothelioma Cells.

IF 1 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Yonago acta medica Pub Date : 2026-05-04 eCollection Date: 2026-05-01 DOI:10.33160/yam.2026.05.007

Tomohiro Osaki, Taichi Kadohata, Miki Yasunaga, Sohta Hishikawa, Yuji Sunden, Kiwamu Takahashi

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引用次数: 0

Abstract

Background: We established persistently passable canine malignant mesothelioma (cMM) cell lines from tumor samples obtained from affected dogs, analyzed their characteristics, and investigated their sensitivity to anticancer drugs and photodynamic therapy (PDT) using 5-aminolevulinic acid hydrochloride (5-ALA).

Methods: Histopathological analyses were performed on SDK cells cultured under two-dimensional (2D) and three-dimensional (3D) conditions, as well as on tumor tissues derived from SDK cells. Cell proliferation in both culture systems and sensitivity to anticancer agents and PDT were assessed.

Results: In 2D culture, SDK cells appeared round to polygonal, with marked variability in size and the presence of pyknotic nuclei. In 3D culture, both large and small cells formed loose clusters with scattered cellular processes. Following subcutaneous transplantation into mice, tumor masses exhibited nodular lesions composed of tumor cells and fibroblasts. Cytokeratin expression was prominent in 3D-cultured cells and tumor tissues but weak in 2D-cultured cells. Vimentin and calretinin were expressed in all cells. SDK cells showed minimal sensitivity to carboplatin but were sensitive to doxorubicin. Notably, SDK cells were also sensitive to 5-ALA-mediated PDT.

Conclusion: SDK cells may serve as a novel cell line for elucidating the pathogenesis of cMM and for developing new therapeutic strategies.

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犬恶性间皮瘤细胞的建立与表征。

背景：我们从患犬的肿瘤样本中建立了犬恶性间皮瘤（cMM）细胞株，分析了它们的特征，并研究了它们对抗癌药物和5-氨基乙酰丙酸盐化物（5-ALA）光动力治疗（PDT）的敏感性。方法：对二维（2D）和三维（3D）条件下培养的SDK细胞以及SDK细胞衍生的肿瘤组织进行组织病理学分析。两种培养系统中的细胞增殖和对抗癌药物和PDT的敏感性进行了评估。结果：在二维培养中，SDK细胞呈圆形至多边形，大小变化明显，细胞核固缩。在三维培养中，大小细胞均形成松散的细胞簇，细胞过程分散。小鼠皮下移植后，肿瘤肿块呈现由肿瘤细胞和成纤维细胞组成的结节状病变。细胞角蛋白在3d培养细胞和肿瘤组织中表达显著，而在2d培养细胞中表达较弱。Vimentin和calretinin在所有细胞中均有表达。SDK细胞对卡铂敏感性极低，但对阿霉素敏感。值得注意的是，SDK细胞对5- ala介导的PDT也很敏感。结论：SDK细胞可作为一种新的细胞系，用于阐明慢性粒细胞白血病的发病机制和开发新的治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Yonago acta medica MEDICINE, RESEARCH & EXPERIMENTAL-

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Yonago Acta Medica (YAM) is an electronic journal specializing in medical sciences, published by Tottori University Medical Press, 86 Nishi-cho, Yonago 683-8503, Japan. The subject areas cover the following: molecular/cell biology; biochemistry; basic medicine; clinical medicine; veterinary medicine; clinical nutrition and food sciences; medical engineering; nursing sciences; laboratory medicine; clinical psychology; medical education. Basically, contributors are limited to members of Tottori University and Tottori University Hospital. Researchers outside the above-mentioned university community may also submit papers on the recommendation of a professor, an associate professor, or a junior associate professor at this university community. Articles are classified into four categories: review articles, original articles, patient reports, and short communications.