Molecular profiling of methicillin-resistant Staphylococcus aureus isolated from healthy pet dogs and their owners in western Iran.

IF 1.9 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2026-04-01 DOI:10.18502/ijm.v18i2.21302

Amir Mohammad Korani, Elham Ahmadi, Safoura Derakhshan

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Abstract

Background and objectives: Growing apprehension surrounds methicillin-resistant Staphylococcus aureus (MRSA) strains. The objective of this investigation was to molecularly profile MRSA strains recovered from asymptomatic companion dogs and their human caretakers in Kermanshah, located in western Iran, marking the inaugural research of its kind within the country.

Materials and methods: During a six-month period, specimens were obtained from the oral cavities and nasal passages of 200 clinically normal pet dogs, as well as their owners. MRSA isolates were identified, DNA was extracted, and characterized via staphylococcal cassette chromosome mec (SCCmec), accessory gene regulator (agr), and staphylococcal protein A (spa) typing, plus detection of mecA and Panton-Valentine leukocidin (PVL) genes.

Results: Eighteen MRSA isolates were identified (12 from dogs, six from owners). All carried mecA. Most had SCCmec type III. Human isolates included one agr II and two agr IV; no dog isolates were agr-positive. Isolates belonged to four spa types: t690 (dogs) and t325, t037, t030 (owners). PVL genes were absent. Isolates from dogs and owners showed type diversity.

Conclusion: This study reveals MRSA epidemiology in healthy dogs and owners in Iran, with low colonization rates. Continuous monitoring is essential to track MRSA circulation, as colonization raises infection risk.

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伊朗西部健康宠物狗及其主人身上分离的耐甲氧西林金黄色葡萄球菌的分子谱分析。

背景和目的：对耐甲氧西林金黄色葡萄球菌（MRSA）菌株的担忧日益增加。这项调查的目的是对伊朗西部克尔曼沙阿（Kermanshah）无症状伴侣犬及其人类照料者身上恢复的MRSA菌株进行分子分析，这是该国同类研究的首次开展。材料和方法：在6个月的时间里，从200只临床正常的宠物狗及其主人的口腔和鼻道中获得标本。对MRSA分离株进行鉴定，提取DNA，通过葡萄球菌盒式染色体mec （SCCmec）、辅助基因调控因子（agr）、葡萄球菌蛋白A （spa）分型，以及检测mecA和pton - valentine leukocidin （PVL）基因进行鉴定。结果：共分离到18株MRSA（12株来自犬，6株来自饲主）。所有人都带着mecA。大多数为SCCmec III型。人类分离株包括1株agr II型和2株agr IV型；犬分离株无agr阳性。分离株属于4种spa类型：t690（犬）和t325、t037、t030（饲主）。PVL基因缺失。犬和饲主分离株表现出类型多样性。结论：本研究揭示了MRSA在伊朗健康狗和主人中的流行病学，其定植率较低。持续监测是追踪MRSA循环的必要条件，因为定植会增加感染风险。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.