Serovar Distribution, Virulence Gene Profiles, and Antimicrobial Resistance of Salmonella Isolated from Chicken Meat from the Broiler Slaughterhouse and Processing Plant in Central Thailand.

IF 2.3 2区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY

Foodborne pathogens and disease Pub Date : 2026-11-01 Epub Date: 2026-05-25 DOI:10.1177/15353141261453452

Wattana Pelyuntha, David Yembilla Yamik, Haemarat Khongkhai, Rungarun Chasuepue, Wichanan Wannasrichan, Kitiya Vongkamjan

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Abstract

Salmonella species are among the most important foodborne pathogens of public health concern, described by the World Health Organization as high-priority foodborne pathogen causing a wide range of infections. Salmonella harbors numerous virulence genes (VGs) and antimicrobial resistance (AMR) mechanisms that contribute to its pathogenicity and resistance to antimicrobial agents. Therefore, this cross-sectional surveillance study aimed to assess the prevalence, serovar distribution, VGs, and phenotypic AMR profiles of Salmonella isolated from chicken meat collected from broiler slaughterhouses and a processing plant. A total of 859 chicken meat samples (666 from three slaughterhouses and 193 from a processing plant) were collected and analyzed for Salmonella. The pathogen was detected in 188 of 859 samples (21.9%), with multiple serovars identified among the isolates. The prevalence was 10.9% in the processing plant and ranged from 9 to 39% in slaughterhouses. Salmonella Agona (72/188; 38.30%) and Salmonella Molade (18/188; 9.57%) were frequently detected. Among VGs, sitC was the most prevalent, detected in 98.95% of the isolates, while cdtB was the least, found in 12.23% of the isolates. Antimicrobial susceptibility was tested by disk diffusion for most antibiotics and broth microdilution for colistin, following CLSI M100 (2021) guidelines. Most strains were resistant to ampicillin, streptomycin, gentamicin, trimethoprim-sulfamethoxazole, and tetracycline, with resistance rates of 58.4%, 50.0%, 33.5%, 31.9%, and 30.3%, respectively. Colistin showed the lowest resistance rate (7.4%). In contrast, the isolates exhibited high susceptibility to amoxicillin-clavulanic acid (88.8%) and ceftriaxone (86.2%). Overall, multidrug resistance (MDR) was observed in 45.21% of the isolates. MDR was found across several serovars, especially Salmonella Agona. The high prevalence and diversity of Salmonella and its phenotypic AMR in chicken meat indicate widespread contamination in slaughterhouses and processing plants, underscoring the need for stronger control measures and improved regulation of antimicrobial use in poultry production.

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泰国中部肉鸡屠宰场和加工厂鸡肉中分离沙门氏菌的血清分布、毒力基因谱和耐药性

沙门氏菌是公共卫生关注的最重要的食源性病原体之一，被世界卫生组织描述为引起广泛感染的高优先级食源性病原体。沙门氏菌含有许多毒力基因（VGs）和抗微生物药物耐药性（AMR）机制，这些机制有助于其致病性和抗微生物药物耐药性。因此，本横断面监测研究旨在评估从肉鸡屠宰场和加工厂收集的鸡肉中分离的沙门氏菌的患病率、血清型分布、VGs和表型AMR谱。总共收集了859份鸡肉样本（666份来自三个屠宰场，193份来自一个加工厂）并对沙门氏菌进行了分析。859份样本中检出病原菌188份（21.9%），分离物中检出多种血清型。加工厂的流行率为10.9%，屠宰场的流行率为9%至39%。高发沙门菌（72/188;38.30%）和Molade沙门菌（18/188;9.57%）。在VGs中，sitC最常见，检出率为98.95%，cdtB最少，检出率为12.23%。按照CLSI M100（2021）指南，采用纸片扩散法检测大多数抗生素，用肉汤微量稀释法检测粘菌素。大多数菌株对氨苄西林、链霉素、庆大霉素、甲氧苄啶-磺胺甲恶唑和四环素耐药，耐药率分别为58.4%、50.0%、33.5%、31.9%和30.3%。粘菌素耐药率最低（7.4%）。菌株对阿莫西林-克拉维酸和头孢曲松的敏感性分别为88.8%和86.2%。总体而言，45.21%的菌株存在多药耐药（MDR）。在几种血清型中发现了耐多药，尤其是沙门氏菌。鸡肉中沙门氏菌的高流行率和多样性及其表型抗菌素耐药性表明屠宰场和加工厂普遍存在污染，强调需要加强控制措施和改进家禽生产中抗菌素使用的监管。

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来源期刊

Foodborne pathogens and disease 医学-食品科技

CiteScore

5.30

自引率

3.60%

发文量

审稿时长

1 months

期刊介绍： Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.