Identification of proteins with altered PTM levels in patients with temporal lobe epilepsy in different regions of the human brain.

Abstract

In this study we have reanalyzed the original proteomic data from studies of hippocampal [DOI: 10.1172/jci.insight.188612] and temporal cortex [DOI: 10.1016/j.expneurol.2025.115361] tissues of patients with temporal lobe epilepsy. The goal was to identify proteins whose levels of post-translational modifications (PTMs) could be altered in this disease. Three datasets (PXD064519, ZMP8KU, and PXD010154) deposited at PRIDE and Texas Data Repository were used. Phosphorylation and N-terminal acetylation were considered as target PTMs. During re-identification of peptides we considered only peptides, for which both variants (with and without PTMs) were identified. A data comparison algorithm independent of experimental data alignment between individual samples was used. As a result, 35 proteins were selected that showed significant changes in phosphorylation levels. For 12 of these proteins, significant changes were recorded in both data sets. Twenty-nine proteins from this list have been linked to epilepsy pathogenesis in the literature.