Orthogonal regulation of protein abundance

IF 14.4 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Trends in Biochemical Sciences Pub Date : 2026-07-01 Epub Date: 2026-05-25 DOI:10.1016/j.tibs.2026.04.013

Wenxin He (何文馨), Mingming Han (韩明明), Wenjing Qiao (乔文静), Cong Gao (高聪), Liming Liu (刘立明), Jing Wu (吴静), Guipeng Hu (胡贵鹏)

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引用次数: 0

Abstract

Precise regulation of protein abundance is essential for cellular function and physiology. Conventional approaches are often limited by insufficient resolution or unintended crosstalk. In contrast, orthogonal control technologies enable programmable and precise modulation of protein abundance while remaining insulated from native networks. In this review, we summarize the development and application of regulation technologies with different orthogonality across multiple levels. Orthogonal transcriptional control primarily involves the design and engineering of orthogonal RNA polymerases and transcription factors; orthogonal translational regulation focuses on advances in genetic codon expansion and post-translational modifications; targeted protein degradation and compartmentalized regulation are also discussed. Finally, we highlight the integration across the different levels described above. This review might bring disruptive insights and conceptual breakthroughs to precision medicine and sustainable biomanufacturing.

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蛋白质丰度的正交调节。

精确调节蛋白质丰度对细胞功能和生理至关重要。传统的方法常常受到分辨率不足或无意的串扰的限制。相比之下，正交控制技术可以实现可编程和精确的蛋白质丰度调制，同时保持与本地网络绝缘。本文综述了不同正交度调控技术在不同水平上的发展和应用。正交转录控制主要涉及正交RNA聚合酶和转录因子的设计和工程；正交翻译调控主要关注基因密码子扩展和翻译后修饰的研究进展；靶蛋白降解和区隔化调控也进行了讨论。最后，我们强调上述不同级别之间的集成。这一综述可能会给精准医疗和可持续生物制造带来颠覆性的见解和概念上的突破。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Trends in Biochemical Sciences 生物-生化与分子生物学

CiteScore

22.90

自引率

0.70%

发文量

148

审稿时长

6-12 weeks

期刊介绍： For over 40 years, Trends in Biochemical Sciences (TIBS) has been a leading publication keeping readers informed about recent advances in all areas of biochemistry and molecular biology. Through monthly, peer-reviewed issues, TIBS covers a wide range of topics, from traditional subjects like protein structure and function to emerging areas in signaling and metabolism. Articles are curated by the Editor and authored by top researchers in their fields, with a focus on moving beyond simple literature summaries to providing novel insights and perspectives. Each issue primarily features concise and timely Reviews and Opinions, supplemented by shorter articles including Spotlights, Forums, and Technology of the Month, as well as impactful pieces like Science & Society and Scientific Life articles.