Estrous synchronization in agoutis (Dasyprocta leporina) using the Ovsynch protocol, followed by natural mating and evaluation of success via offspring birth

Abstract

Research on estrous synchronization and/or induction in the Dasyprocta species remains limited and no previous studies have confirmed reproductive success through offspring birth following hormonal synchronization protocols. This study aimed to evaluate the use of the Ovsynch protocol for estrous synchronization in Dasyprocta leporina, followed by natural mating and assessment of reproductive success via parturition. Five female agoutis were synchronized by firstly administering subcutaneous injections of 40 µg gonadorelin acetate (Fertiline®), followed seven days later by intramuscular injections of 2.5 mg dinoprost tromethamine (Lutalyse®) and a second set of subcutaneous injections of 40 µg gonadorelin acetate (Fertiline®) two days after. A proven male was introduced at a time corresponding to the expected onset of estrus based on previously established data. Animals were monitored daily and reproductive success was determined by offspring birth. Three of the five females (60%) produced offspring, including two single births and one twin birth. Birth weights ranged from 222 to 250 g and estimated gestational lengths (107–120 days) were consistent with published values for the species. These findings provide preliminary evidence that the Ovsynch protocol may support successful reproduction in D. leporina under low-intervention conditions. However, due to the small sample size, absence of a control group and lack of direct monitoring, the results should be interpreted cautiously. This study contributes baseline data for the development of assisted reproductive techniques in this species.