[Establishing the species of myocardial microparticles by human total IgG].

Q4 Medicine
A A Gusarov, V L Sidorov, N E Surikova, O D Yagmurov, K T Momynaliev
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引用次数: 0

Abstract

Objective: Establishing the species origin of microparticles of striated cardiac muscle tissue according to human total IgG by the method of quantitative solid-phase enzyme-linked immunosorbent assay using the IgG-total-ELISA-HEMA kit.

Material and methods: Myocardial microparticles taken from the corpses of eight people. The samples were analyzed using the quantitative solid-phase ELISA method using the IgG-total-ELISA-HEMA test kit.

Results: The optical density and concentration of total IgG in whole extracts were determined, and the optimal values of the minimum and maximum calibration samples were obtained: 0.082±0.015-2.581±0.017.

Conclusion: The obtained data can be used by experts of forensic biological units to establish the species affiliation of microparticles of striated cardiac muscle tissue found on trauma tools using the quantitative solid-phase ELISA method for human total IgG.

[用人总IgG建立心肌微粒的种类]。
目的:采用IgG-total- elisa - hema试剂盒,采用固相酶联免疫吸附法定量测定人总IgG,建立心肌组织横纹肌微粒的种源。材料与方法:取8人尸体心肌微粒。采用IgG-total-ELISA-HEMA检测试剂盒,采用定量固相ELISA法对样品进行分析。结果:测定了全提取物中总IgG的光密度和浓度,得到了最小和最大定标样品的最佳值:0.082±0.015-2.581±0.017。结论:所得数据可用于法医生物单位专家利用人总IgG定量固相ELISA法建立创伤工具上发现的心肌组织条纹微粒的种类归属。
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来源期刊
Sudebno-Meditsinskaya Ekspertisa
Sudebno-Meditsinskaya Ekspertisa Medicine-Pathology and Forensic Medicine
CiteScore
0.60
自引率
0.00%
发文量
70
期刊介绍: The journal is concerned with the theory and practice of forensic medicine - the problems of thanatology, traumatology, toxicology, serology, forensic obstetrics, forensic dentistry, forensic psychiatry, forensic chemistry, physicotechnical methods of investigation, history of forensic medicine and some problems of criminology and legal laws related to forensic medicine. It publishes original studies by Russian authors, casuistry surveys, abstracts and reviews of Russian and foreign literature, scientific information, reports on scientific conferences.
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