Bioprospecting of waste dump site reveals degradation potential among Bacillus cereus KRS102 and Bacillus tropicus KRS236 against UV-treated polypropylene

IF 4.2 4区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biodegradation Pub Date : 2026-05-15 DOI:10.1007/s10532-026-10311-z

Swati Rani, Himalaya Panwar, Kartikey Kumar Gupta

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Abstract

The present work aims to achieve degradation of UV treated Polypropylene (PP) by utilizing the capabilities of two bacterial strains designated as KRS102 and KRS236. Both strains were isolated from a waste dump site in Rishikesh (India), identified through 16S rDNA sequencing and compared for PP degradation ability. Detection of biosurfactant production and hydrophobicity assessment validated biodegradation potential of the isolated strains while 16S rDNA secondary structure prediction using RNAfold confirmed their thermodynamic stability. The biodegradation of PP films in terms of percent weight loss by Bacillus cereus KRS102 and Bacillus tropicus KRS236 was found to be 3.4 ± 0.20% and 2.9 ± 0.18%, respectively following 90 days of incubation period. Strain KRS102 exhibited faster depolymerization dynamics than KRS236, as indicated by its higher biomass (protein content: 9.64 ± 3.3 µg mL⁻¹), greater degradation rate constant (0.000384 day⁻¹), and shorter half-life (1805 days) as determined by kinetic modeling. EDX analysis indicated oxidative cleavage of the polymer chains. The KRS102-treated film showed a marked increase in oxygen content (27.74%) and a corresponding decrease in carbon (72.26%), reflecting extensive oxidative modification. In contrast, the control PP film exhibited minimal oxygen (0.94%) and was predominantly composed of carbon (99.10%). The FTIR study revealed changed functional group intensities and an increase in the carbonyl index. These alterations show that the biofilm matrix promotes substrate enzymatic interactions and biochemical transformations, indicating increased metabolic activity in the microbial biofilm. GC–MS and SEM analysis corroborated these findings through degradation intermediates and visible surface alterations respectively. Overall, Bacillus cereus KRS102 exhibited higher biodegradation efficiency than Bacillus tropicus KRS236, highlighting its potential as environment friendly PP degrader.

Graphical abstract

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对垃圾场进行生物勘探，发现蜡样芽孢杆菌KRS102和热带芽孢杆菌KRS236对紫外光处理聚丙烯具有降解潜力

本研究旨在利用KRS102和KRS236两种细菌菌株的能力，实现紫外线处理聚丙烯（PP）的降解。这两株菌株均分离自印度Rishikesh的一个垃圾场，通过16S rDNA测序鉴定并比较了PP降解能力。生物表面活性剂产量检测和疏水性评价验证了分离菌株的生物降解潜力，利用RNAfold进行16S rDNA二级结构预测证实了其热力学稳定性。经过90 d的培养，蜡样芽孢杆菌KRS102和热带芽孢杆菌KRS236对PP膜的生物降解率分别为3.4±0.20%和2.9±0.18%。菌株KRS102比KRS236表现出更快的解聚动力学，这表明它具有更高的生物量（蛋白质含量：9.64±3.3µg mL毒血症），更大的降解速率常数（0.000384天毒血症）和更短的半衰期（1805天）。EDX分析表明聚合物链发生氧化裂解。经krs102处理后的膜氧含量显著增加（27.74%），碳含量显著降低（72.26%），反映了广泛的氧化修饰。相比之下，对照PP膜氧含量最低（0.94%），主要由碳组成（99.10%）。FTIR研究显示，官能团强度发生变化，羰基指数增加。这些变化表明，生物膜基质促进底物酶相互作用和生化转化，表明微生物生物膜的代谢活性增加。GC-MS和SEM分析分别通过降解中间体和可见的表面变化证实了这些发现。总体而言，蜡样芽孢杆菌KRS102的生物降解效率高于热带芽孢杆菌KRS236，凸显了其作为环境友好型PP降解剂的潜力。图形抽象此图像的替代文本可能是使用AI生成的。

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来源期刊

Biodegradation 工程技术-生物工程与应用微生物

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Biodegradation publishes papers, reviews and mini-reviews on the biotransformation, mineralization, detoxification, recycling, amelioration or treatment of chemicals or waste materials by naturally-occurring microbial strains, microbial associations, or recombinant organisms. Coverage spans a range of topics, including Biochemistry of biodegradative pathways; Genetics of biodegradative organisms and development of recombinant biodegrading organisms; Molecular biology-based studies of biodegradative microbial communities; Enhancement of naturally-occurring biodegradative properties and activities. Also featured are novel applications of biodegradation and biotransformation technology, to soil, water, sewage, heavy metals and radionuclides, organohalogens, high-COD wastes, straight-, branched-chain and aromatic hydrocarbons; Coverage extends to design and scale-up of laboratory processes and bioreactor systems. Also offered are papers on economic and legal aspects of biological treatment of waste.