Lan Yang, Jie Li, Jia Dai, Jianfang Liu, Demeng Tan, Nannan Wu, Tongyu Zhu, Mengjun Cheng
{"title":"KP-C01: A Novel Phage with Tail-Associated Depolymerase Activity Against KL102 Carbapenem-Resistant <i>Klebsiella pneumoniae</i>.","authors":"Lan Yang, Jie Li, Jia Dai, Jianfang Liu, Demeng Tan, Nannan Wu, Tongyu Zhu, Mengjun Cheng","doi":"10.1177/26416549251406409","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The emergence and spread of carbapenem-resistant <i>Klebsiella pneumoniae</i> (CRKP) represent a major threat to global public health, necessitating effective therapeutic strategies to prevent infection.</p><p><strong>Materials and methods: </strong>A novel bacteriophage, φKP-C01, was isolated and characterized using ST307<sup>KL102</sup> CRKP as the host.</p><p><strong>Results: </strong>One-step growth curve analysis and complete genome sequencing demonstrated φKP-C01 to be a highly efficient lytic phage. The absence of genes encoding virulence factors, drug resistance determinants, or lysogeny-related functions indicated its potential safety for therapeutic applications. Bioinformatic analysis identified <i>orf5</i> as encoding Depo-C01, a putative tail fiber protein with predicted depolymerase activity. To validate this function, <i>orf5</i> was cloned, and recombinant Depo-C01 was expressed and purified. Depolymerase activity specifically targeting the KL102 capsular polysaccharide of the CRKP host was confirmed via spot assay and transmission electron microscopy.</p><p><strong>Conclusion: </strong>Lytic phage φKP-C01 and its depolymerase Depo-C01 show strong potential for phage-based or enzymatic strategies targeting CRKP infections.</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"7 1","pages":"35-42"},"PeriodicalIF":1.8000,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13089262/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"PHAGE (New Rochelle, N.Y.)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1177/26416549251406409","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/3/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Background: The emergence and spread of carbapenem-resistant Klebsiella pneumoniae (CRKP) represent a major threat to global public health, necessitating effective therapeutic strategies to prevent infection.
Materials and methods: A novel bacteriophage, φKP-C01, was isolated and characterized using ST307KL102 CRKP as the host.
Results: One-step growth curve analysis and complete genome sequencing demonstrated φKP-C01 to be a highly efficient lytic phage. The absence of genes encoding virulence factors, drug resistance determinants, or lysogeny-related functions indicated its potential safety for therapeutic applications. Bioinformatic analysis identified orf5 as encoding Depo-C01, a putative tail fiber protein with predicted depolymerase activity. To validate this function, orf5 was cloned, and recombinant Depo-C01 was expressed and purified. Depolymerase activity specifically targeting the KL102 capsular polysaccharide of the CRKP host was confirmed via spot assay and transmission electron microscopy.
Conclusion: Lytic phage φKP-C01 and its depolymerase Depo-C01 show strong potential for phage-based or enzymatic strategies targeting CRKP infections.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
