Marsdenia tenacissima Extract Affects the Proliferation, Apoptosis, and Migration of Oral Squamous Cell Carcinoma Cells Through hsa_circular_0003645/microRNA-335

IF 0.4 4区医学 Q4 PHARMACOLOGY & PHARMACY

Indian Journal of Pharmaceutical Sciences Pub Date : 2024-01-01 DOI:10.36468/pharmaceutical-sciences.1344

Lixin Wang, Jin Fang

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引用次数: 0

Abstract

Numerous papers stated that Marsdenia tenacissima extract possessed tumor-suppression properties. Herein, we aimed to investigate the influence and underlying mechanism of Marsdenia tenacissima extract on oral squamous cell carcinoma. After being cultured, CAL-27 cells were treated with various doses of Marsdenia tenacissima extract. Proliferation, apoptosis, and migration were assessed using cell counting kit-8, clone formation, scratch, and flow cytometry assays. Western blot detected protein levels. Quantitative reverse transcription polymerase chain reaction method analyzed hsa_circular_0003645 and microRNA-335 expression. Their interaction was validated using dual-luciferase reporter gene experiment. 1.0 and 1.5 mg/ml Marsdenia tenacissima extract or si-hsa_circular_0003645 could suppress CAL-27 cell proliferation, migration, N-cadherin, and hsa_circ_0003645, whereas increase apoptosis, E-cadherin, and microRNA-335. Furthermore, the upregulation of hsa_circular_0003645 might abolish Marsdenia tenacissima extract exposure-mediated oral squamous cell carcinoma cell proliferation and migration inhibition and apoptosis promotion. Besides, hsa_circular_0003645 could target microRNA-335. Marsdenia tenacissima extract treatment might hinder oral squamous cell carcinoma progression via regulating hsa_circular_0003645 and microRNA-335.

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马蹄铁提取物通过hsa_circular_0003645/microRNA-335影响口腔鳞状细胞癌细胞的增殖、凋亡和迁移

大量文献报道马尾草提取物具有抑瘤作用。本研究旨在探讨马尾草提取物对口腔鳞状细胞癌的影响及其机制。CAL-27细胞培养后，用不同剂量的马蹄草提取物处理。使用细胞计数试剂盒-8、克隆形成、划痕和流式细胞术检测增殖、凋亡和迁移。Western blot检测蛋白水平。定量逆转录聚合酶链反应法分析hsa_circular_0003645和microRNA-335的表达。通过双荧光素酶报告基因实验验证了它们的相互作用。1.0和1.5 mg/ml麻藤提取物或si- hsa_cirar_0003645均能抑制CAL-27细胞的增殖、迁移、N-cadherin和hsa_cirar_0003645，而增加凋亡、E-cadherin和microRNA-335。此外，hsa_circular_0003645的上调可能会消除马齿苋提取物暴露介导的口腔鳞状细胞癌细胞增殖、迁移抑制和凋亡促进作用。此外，hsa_circular_0003645可以靶向microRNA-335。马齿苋提取物可能通过调节hsa_circular_0003645和microRNA-335抑制口腔鳞状细胞癌的进展。

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来源期刊

Indian Journal of Pharmaceutical Sciences PHARMACOLOGY & PHARMACY-

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： The Indian Journal of Pharmaceutical Sciences (IJPS) is a bi-monthly Journal, which publishes original research work that contributes significantly to further the scientific knowledge in Pharmaceutical Sciences (Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy and Phytochemistry, Pharmacology and Therapeutics, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Pharmacovigilance, Pharmacoepidemiology, Pharmacoeconomics, Drug Information, Patient Counselling, Adverse Drug Reactions Monitoring, Medication Errors, Medication Optimization, Medication Therapy Management, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest). The Journal publishes original research work either as a Full Research Paper or as a Short Communication. Review Articles on current topics in Pharmaceutical Sciences are also considered for publication by the Journal.