outtuynia cordatapolysaccharide attenuates LPS-induced lung injury by inhibiting the HMGB3/JNK pathway through upregulation of miR-200b-3p

IF 0.8 4区医学 Q4 INTEGRATIVE & COMPLEMENTARY MEDICINE

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas Pub Date : 2025-09-30 DOI:10.37360/blacpma.25.24.5.55

Wei Hong Liu, Li Yan Yin, Bao Gang Wang

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Abstract

The aim was toinvestigate the therapeutic effects and molecular mechanisms of Houttuynia cordatapolysaccharide (HCP) on LPS-induced lung injury.Effects were analyzed by HE, TUNEL, ELISA, MTT, and flow cytometry. Roles and mechanisms of HCP, miR-200b-3p, HMGB3, and JNK were studied by RT-qPCR, Western Blot, transfection, and dual luciferase reporter gene.HCP treatment effectively ameliorated LPS-induced lung injury in mice and TC-1 cells and was able to inhibit inflammatory factors as well as apoptosis with an effect close to that of dexamethasone-positive control. miR-200b-3p expression was down-regulated in LPS-induced lung injury and up-regulated by HCP treatment, and miR-200b-3p inhibitors reduced the therapeutic effects of HCP. miR-200b-3p targeted and negatively regulated HMGB3. HMGB3 was able to activate the JNK pathway to suppress the therapeutic effects of HCP on lung cell injury.HCP attenuates LPS-induced lung injury by upregulating miR-200b-3p to inhibit the HMGB3/JNK pathway.

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大黄多糖通过上调miR-200b-3p抑制HMGB3/JNK通路，减轻lps诱导的肺损伤

目的探讨鱼腥草多糖（HCP）对lps诱导的大鼠肺损伤的治疗作用及其分子机制。通过HE、TUNEL、ELISA、MTT和流式细胞术分析效果。采用RT-qPCR、Western Blot、转染、双荧光素酶报告基因等方法研究HCP、miR-200b-3p、HMGB3、JNK的作用及机制。HCP治疗可有效改善lps诱导的小鼠和TC-1细胞的肺损伤，并能抑制炎症因子和细胞凋亡，其作用与地塞米松阳性对照接近。miR-200b-3p表达在lps诱导的肺损伤中下调，在HCP治疗中上调，miR-200b-3p抑制剂降低了HCP的治疗效果。miR-200b-3p靶向并负调控HMGB3。HMGB3能够激活JNK通路，抑制HCP对肺细胞损伤的治疗作用。HCP通过上调miR-200b-3p抑制HMGB3/JNK通路来减弱lps诱导的肺损伤。

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来源期刊

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas PHARMACOLOGY & PHARMACY-

CiteScore

1.30

自引率

14.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas (BLACPMA), [Latin American and Caribbean Bulletin of Medicinal and Aromatic Plants]; currently edited by the publishing house MS-Editions, is a bi-monthly international publication that publishes original peerreviewed research in the field of medicinal and aromatic plants, with nearly 20 years of experience. BLACPMA is a scientific journal that publishes two types of articles: Reviews (only in English) and Original Articles (Spanish or English), its main lines of action being agronomy, anthropology and ethnobotany, industrial applications, botany, quality and standardization, ecology and biodiversity, pharmacology, phytochemistry, pharmacognosy, regulatory and legislative aspects. While all areas of medicinal plants are welcome and the experimental approaches used can be broad and interdisciplinary; other areas of research that are not mentioned depend on the Editorial Committee for their acceptance.