Cav3.3-mediated endochondral ossification in a three-dimensional bioprinted GelMA hydrogel

IF 7.9 1区医学 Q1 ENGINEERING, BIOMEDICAL

Bio-Design and Manufacturing Pub Date : 2024-11-01 DOI:10.1007/s42242-024-00287-1

Zhi Wang, Xin Wang, Yang Huang, Junjun Yang, Zu Wan, Zhenlan Fu, Xiaoyuan Gong, Guangxing Chen, Liu Yang

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引用次数: 4

Abstract

软骨内成骨软骨内成骨 (EO) 是生长板组织内介导长骨发育的重要生物学过程. 骨类器官能够模拟天然生长板中组织结构与细胞间通信的生理功能, 是一种潜在的长骨发育研究模型. 但如何有效模拟EO过程一直是骨类器官构建中尚未解决的核心难题. 为了模拟EO这一有序的矿化过程, 本文首次揭示了T型电压依赖性钙通道 (T-VDCC) 的Ca v 3.3亚型在EO过程中主要表达于软骨细胞, 并且与软骨细胞的肥大分化呈负相关这一生物学机制, 并基于此机制设计了负载shCa v 3.3 ATDC5细胞的明胶甲基丙烯酰 (GelMA) 水凝胶生物墨水, 且进一步评估了这种生物墨水的生物打印性能. 最后, 本研究通过负载Ca v 3.3敲低的软骨细胞构建了GelMA水凝胶模型, 成功在体外模拟了EO过程, 为构建分层有序矿化的骨类器官提供了一种新型的生物墨水. 因此, 本研究不仅为骨类器官构建中的EO进程调控提供了潜在的研究靶点, 也为模拟EO过程提供了新的策略.

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三维生物打印凝胶中cav3.3介导的软骨内成骨

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来源期刊

Bio-Design and Manufacturing Materials Science-Materials Science (miscellaneous)

CiteScore

13.30

自引率

7.60%

发文量

148

期刊介绍： Bio-Design and Manufacturing reports new research, new technology and new applications in the field of biomanufacturing, especially 3D bioprinting. Topics of Bio-Design and Manufacturing cover tissue engineering, regenerative medicine, mechanical devices from the perspectives of materials, biology, medicine and mechanical engineering, with a focus on manufacturing science and technology to fulfil the requirement of bio-design.