Efficient mild depolymerization of polyester plastics accomplished by engineered PETase via directed evolution of flexible loops

IF 6.9 2区综合性期刊 Q1 CHEMISTRY, MULTIDISCIPLINARY

Cell Reports Physical Science Pub Date : 2024-11-01 DOI:10.1016/j.xcrp.2024.102295

Yidi Liu, Huanliu Lin, Zhisheng Wei, S. J. Bai, Sheng Chen, Jing Wu, Zhanzhi Liu

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引用次数: 4

Abstract

Summary

Due to inadequate disposal and recycling of polyethylene terephthalate (PET) plastic products, there has been growing concern about the severe environmental consequences. Biocatalytic degradation of PET has emerged as a promising solution. The PET hydrolase IsPETase from Ideonella sakaiensis exhibits distinctive substrate activity at mild temperatures, but its practical application is limited by restricted catalytic efficiency. In this study, the highly flexible loops of IsPETase are targeted for engineering after a detailed structural analysis. A customized dual-fluorescence high-throughput assay is then developed to facilitate the directed evolution of these selected loops in order to enhance IsPETase's catalytic performance, specifically at mild temperatures. Through implementation of this system, the evolved IsPETase mutant M4-Q demonstrates exceptional PET biodegradation activity at mild temperatures and advantages in degrading various aliphatic-aromatic copolyesters. These findings offer innovative insights into the enzymatic degradation of PET and other polyester plastic waste.

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通过柔性环的定向进化，工程PETase完成了聚酯塑料的高效温和解聚

由于聚乙烯对苯二甲酸乙二醇酯（PET）塑料制品的处理和回收不足，人们越来越关注其严重的环境后果。生物催化降解PET已成为一种很有前途的解决方案。酒井Ideonella sakaiensis的PET水解酶IsPETase在温和温度下表现出独特的底物活性，但其实际应用受到催化效率的限制。在本研究中，IsPETase的高柔性环在经过详细的结构分析后，成为工程化的目标。然后开发了定制的双荧光高通量分析，以促进这些选定环的定向进化，以增强IsPETase的催化性能，特别是在温和的温度下。通过该系统的实施，进化的IsPETase突变体M4-Q在温和的温度下表现出优异的PET生物降解活性，并且在降解各种脂肪族-芳香族共聚酯方面具有优势。这些发现为PET和其他聚酯塑料废物的酶降解提供了创新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Reports Physical Science Energy-Energy (all)

CiteScore

11.40

自引率

2.20%

发文量

388

审稿时长

62 days

期刊介绍： Cell Reports Physical Science, a premium open-access journal from Cell Press, features high-quality, cutting-edge research spanning the physical sciences. It serves as an open forum fostering collaboration among physical scientists while championing open science principles. Published works must signify significant advancements in fundamental insight or technological applications within fields such as chemistry, physics, materials science, energy science, engineering, and related interdisciplinary studies. In addition to longer articles, the journal considers impactful short-form reports and short reviews covering recent literature in emerging fields. Continually adapting to the evolving open science landscape, the journal reviews its policies to align with community consensus and best practices.