In vitro cytotoxic activity of Sansevieria trifasciata against various cancer cell lines

IF 0.6 4区医学 Q4 PHARMACOLOGY & PHARMACY

Tropical Journal of Pharmaceutical Research Pub Date : 2024-04-14 DOI:10.4314/tjpr.v23i3.2

Sheryar Afzal, Yuan Seng Wu, Chan Zelynn, V Appalaraju

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引用次数: 2

Abstract

Purpose: To determine the in vitro anticancer properties of Sansevieria trifasciata extracts. Methods: The cytotoxic activity of n-hexane, ethyl acetate and methanol leaf extracts at different concentrations was assessed against Vero, pancreatic (AsPC-1), lung (A549), esophageal squamous carcinoma (KYSE 150) and rhabdomyosarcoma (RD) cancer cell lines using MTT assay. The apoptosis-inducing potential of selected S. trifasciata leaf extract was determined using a caspase 3/7 assay kit. Results: Ethyl acetate extract of S. trifasciata leaf showed significant anticancer activity against RD cells and methanol extract against KYSE cells (p < 0.05). Ethyl acetate and methanol extract possessed higher toxicity towards RD and KYSE 150 cell lines and exhibited relatively lower toxicity towards Vero normal cells. Ethyl acetate extract-treated RD cells and KYSE 150 cancer cells had more typical apoptotic morphologic features when compared to normal cells. Conclusion: Sansevieria trifasciata possesses anticancer activity and provides new insights for future molecular work on investigating its utilization in cancer treatment.

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；体外<；/i>；三叶草<；/i>；对多种癌细胞的细胞毒活性

目的：研究三叶藤提取物的体外抗癌作用。方法：采用MTT法测定不同浓度正己烷、乙酸乙酯和甲醇叶提取物对Vero、胰腺（AsPC-1）、肺（A549）、食管鳞癌（KYSE 150）和横纹肌肉瘤（RD）癌细胞的细胞毒活性。采用caspase 3/7检测试剂盒检测三叶草提取物诱导细胞凋亡的能力。结果：三叶草叶乙酸乙酯提取物对RD细胞和甲醇提取物对KYSE细胞有显著的抗肿瘤活性（p < 0.05）。乙酸乙酯和甲醇提取物对RD和KYSE 150细胞株的毒性较高，对Vero正常细胞的毒性相对较低。与正常细胞相比，乙酸乙酯提取物处理的RD细胞和KYSE 150癌细胞具有更典型的凋亡形态学特征。结论：三叶参具有抗癌活性，为进一步研究其在肿瘤治疗中的应用提供了新的思路。

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来源期刊

Tropical Journal of Pharmaceutical Research 医学-药学

CiteScore

1.00

自引率

33.30%

发文量

490

审稿时长

4-8 weeks

期刊介绍： We seek to encourage pharmaceutical and allied research of tropical and international relevance and to foster multidisciplinary research and collaboration among scientists, the pharmaceutical industry and the healthcare professionals. We publish articles in pharmaceutical sciences and related disciplines (including biotechnology, cell and molecular biology, drug utilization including adverse drug events, medical and other life sciences, and related engineering fields). Although primarily devoted to original research papers, we welcome reviews on current topics of special interest and relevance.

<i>In vitro</i> cytotoxic activity of <i>Sansevieria trifasciata</i> against various cancer cell lines

Abstract