Evaluation of active components and pharmacological activities by UPLC-Q/TOF-MS and network pharmacology in Lentinula edodes and Lyophyllum decastes

IF 4.5 3区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Quality Assurance and Safety of Crops & Foods Pub Date : 2024-12-21 DOI:10.15586/qas.v16i4.1510

Yingnan Zhang, Gaoxing Ma, Fei Pei, Ning Ma, Anxiang Su, Qiuhui Hu, Meng Wang

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引用次数: 3

Abstract

Lentinula edodes (L. edodes) is a premium edible fungus cultivated extensively throughout China, and Lyophyllum decastes (L. decastes) is a wild edible fungus with a high degree of industrial-level cultivation in China. Regarding the edible value of the two fungi, research on their medicinal properties is relatively scarce. In this study, we utilized ultra-high performance liquid chromatography with quadrupole and time-of-flight mass spectrometry to analyze comprehensively the chemical composition of both fungi. In all, 120 and 144 chemical components were identified in L. edodes and L. decastes, respectively. Subsequently, we elaborated active components, potential biological activities, and therapeutic targets associated with both fungi by using network pharmacology. The results showed that seven active components in L. edodes and nine active components in L. decastes could act on common targets, such as AKT serine/threonine kinase1 and peroxisome proliferator-activated receptor gamma, thereby regulating signaling pathways, such as epidermal growth factor receptor and mitogen-activated protein kinase, to affect the body. In addition, these components affect immune regulation and inhibition of liver cancer. Finally, we used cell experiments to verify their activities. Our findings provided a reliable foundation for future research aimed at harnessing the bioactivities of L. edodes and L. decastes, thereby offering novel strategies for their utilization in the field of functional foods and natural medicine.

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UPLC-Q/TOF-MS和网络药理学评价香菇和Lyophyllum decastes<的有效成分和药理活性

香菇（Lentinula edodes， L. edodes）是中国广泛种植的优质食用菌，Lyophyllum decdecs （L. decdecs）是中国工业化程度较高的野生食用菌。关于这两种真菌的食用价值，对其药用特性的研究相对较少。在本研究中，我们利用四极柱超高效液相色谱和飞行时间质谱对这两种真菌的化学成分进行了综合分析。共鉴定出120种化学成分，鉴定出144种化学成分。随后，我们利用网络药理学阐述了这两种真菌的活性成分、潜在的生物活性和治疗靶点。结果表明，白羊草中的7种活性成分和白羊草中的9种活性成分可作用于AKT丝氨酸/苏氨酸激酶e1和过氧化物酶体增殖物活化受体γ等共同靶点，从而调节表皮生长因子受体和丝裂原活化蛋白激酶等信号通路，影响机体。此外，这些成分还影响肝癌的免疫调节和抑制。最后，我们用细胞实验来验证它们的活动。本研究结果为进一步研究香菇和腐烂乳杆菌的生物活性提供了可靠的基础，从而为其在功能食品和天然药物领域的应用提供了新的策略。

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来源期刊

Quality Assurance and Safety of Crops & Foods FOOD SCIENCE & TECHNOLOGY-

CiteScore

4.60

自引率

7.50%

发文量

审稿时长

1 months

期刊介绍： ''Quality Assurance and Safety of Crops & Foods'' is an international peer-reviewed journal publishing research and review papers associated with the quality and safety of food and food sources including cereals, grains, oilseeds, fruits, root crops and animal sources. It targets both primary materials and their conversion to human foods. There is a strong focus on the development and application of new analytical tools and their potential for quality assessment, assurance, control and safety. The scope includes issues of risk assessment, traceability, authenticity, food security and socio-economic impacts. Manuscripts presenting novel data and information that are likely to significantly contribute to scientific knowledge in areas of food quality and safety will be considered. ''Quality Assurance and Safety of Crops & Foods'' provides a forum for all those working in the specialist field of food quality and safety to report on the progress and outcomes of their research.

Evaluation of active components and pharmacological activities by UPLC-Q/TOF-MS and network pharmacology in <i>Lentinula edodes</i> and <i>Lyophyllum decastes</i>

Abstract