Deep Eutectic Solvent Can Inhibit the Proliferation of Hepatocellular Carcinoma Cells in Many Ways

Abstract

研究目的：研究以氯化胆碱(CHCL)和维生素C(VITC)为组成成分所形成的深共熔溶剂(DES)对肿瘤细胞增殖能力的影响研究。方法：用DES混合液处理细胞24 h后，采用MTT实验检测细胞的增殖能力；采用凋亡坏死试剂盒检测细胞中的凋亡水平；采用Western blot检测细胞相关蛋白的表达水平；采用透射电镜观察DES对细胞的细胞膜及细胞器等各方面的影响。研究结果：DES对细胞具有毒性作用；DES促进肝癌细胞的凋亡坏死进而抑制细胞增殖；DES通过抑制PI3K-AKT通路进而抑制细胞的增殖。研究结论：深共熔溶剂可以从多种途径抑制肿瘤细胞的增殖。Objective: To investigate the effect of deep eutectic solvent (DES) formed with choline chloride (CHCL) and vitamin C (VITC) as components on the proliferation ability of tumor cells. Methods: After treating the cells with DES mixture for 24 h, the proliferation ability of the cells was detected by MTT assay. Apoptosis level was detected by apoptotic necrosis kit. The expression levels of related proteins were detected by Western blot. The effects of DES on cell membranes and organelles were observed by transmission electron microscopy. The results showed that DES had toxic effect on cells. DES promoted apoptosis and necrosis of hepatocellular carcinoma cells and inhibited cell proliferation. DES inhibits cell proliferation by inhibiting PI3K-AKT pathway. Conclusion: Deep eutectic solvent can inhibit the proliferation of tumor cells in many ways.