FeS2 nanosheets as mimetic peroxidase for sensitive colorimetric detection of biothiols

IF 2.6 4区化学 Q3 CHEMISTRY, MULTIDISCIPLINARY

Bulletin of the Chemical Society of Ethiopia Pub Date : 2025-07-22 DOI:10.4314/bcse.v39i9.4

Shangying Qin, Li Huang, Xin Shu, Jin Lin Yang, Shiqi Cheng, Yilin Wang

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引用次数: 1

Abstract

The preparation and application of nanomaterials with enzyme-like activity have attracted widespread attention from researchers. Herein, we prepared FeS2 nanosheets (FeS2 NSs) with peroxidase mimicking behavior through a simple hydrothermal pathway. Then, a FeS2 NSs-based colorimetric assay for biothiols detection was established. On one hand, FeS2 NSs could catalyze H2O2 to oxidize colorless 3,3',5,5'-tetramethylbenzidine (TMB) to blue oxidized product (ox-TMB) with a characteristic peak at 652 nm. On the other hand, when a biothiol compound including cysteine (CySH), homocysteine (HcySH) and glutathione (GSH) was present, ox-TMB would be reduced, resulting in lighter blue and lower absorbance. Suggesting that the change of absorbance could be used for quantitative detection of biothiols. Taking HcySH as an example, several experimental parameters including pH, incubation time, and TMB concentration were optimized. Under the conditions of pH 4.5, incubation time of 50 min, and TMB of 0.4 mM, the limit of detection for HcySH, CySH and GSH was as low as 0.67, 0.59 and 0.36 μM, respectively. To verify its possible application, this method was further applied for the determination of HcySH in real serum samples, and good recoveries of 95.0 to 103.8% were obtained. KEY WORDS: Nanosheets, Peroxidase‐like activity, Spectrophotometric, Determination Bull. Chem. Soc. Ethiop. 2025, 39(9), 1699-1711. DOI: https://dx.doi.org/10.4314/bcse.v39i9.4

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FeS2纳米片作为模拟过氧化物酶用于生物硫醇的灵敏比色检测

具有类酶活性的纳米材料的制备和应用引起了研究者的广泛关注。本文通过简单的水热途径制备了具有过氧化物酶模拟行为的FeS2纳米片（FeS2 NSs）。建立了基于FeS2 nss的生物硫醇比色检测方法。一方面，FeS2 NSs可以催化H2O2将无色的3,3',5,5'-四甲基联苯胺（TMB）氧化为蓝色氧化产物（x-TMB），其特征峰位于652nm处；另一方面，当含有半胱氨酸（CySH）、同型半胱氨酸（HcySH）和谷胱甘肽（GSH）的生物硫醇化合物存在时，x- tmb会减少，导致蓝色较浅，吸光度较低。提示吸光度的变化可用于生物硫醇的定量检测。以HcySH为例，对pH、孵育时间、TMB浓度等实验参数进行优化。在pH为4.5、孵育时间为50 min、TMB为0.4 mM的条件下，HcySH、CySH和GSH的检出限分别为0.67、0.59和0.36 μM。为验证该方法的可行性，进一步将该方法应用于实际血清样品中HcySH的测定，回收率为95.0 ~ 103.8%。关键词：纳米片，过氧化物酶样活性，分光光度法，测定化学。Soc。埃塞俄比亚。2025,39(9),1699-1711。DOI: https://dx.doi.org/10.4314/bcse.v39i9.4

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来源期刊

Bulletin of the Chemical Society of Ethiopia 化学-化学综合

CiteScore

2.20

自引率

8.30%

发文量

113

审稿时长

6-12 weeks

期刊介绍： The Bulletin of the Chemical Society of Ethiopia (BCSE) is a triannual publication of the Chemical Society of Ethiopia. The BCSE is an open access and peer reviewed journal. The BCSE invites contributions in any field of basic and applied chemistry.