Isolation, Characterization, and Biopreservation of Lactobacillus brevis DN-1 to Inhibit Mold and Remove Aflatoxin B1 in Peanut and Sunflower Cakes

IF 4.5 2区农林科学 Q1 AGRONOMY

Agriculture-Basel Pub Date : 2024-04-29 DOI:10.3390/agriculture14050698

Xiaoni Wang, Siyuan Wang, Junzhao Xu, Baiyila Wu, Zongfu Hu, Huaxin Niu

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引用次数: 0

Abstract

Aflatoxin B1 (AFB1) is the most toxic mycotoxin and is widespread in moldy feed. The use of biological removal methods to reduce AFB1 has become a research hotspot. This study aimed to isolate lactic acid bacteria (LAB) capable of removing AFB1 from moldy feeds and assessed the removal capacity under various environmental conditions. A strain named Lactobacillus brevis DN-1 was isolated from feed samples and showed 71.38% AFB1 percent removal. Furthermore, DN-1 showed good antifungal activity against Aspergillus flavus BNCC336156 and Aspergillus parasiticus BNCC335939. The optimum growth temperature and pH of DN-1 were 37 °C and 6.0, respectively, and DN-1 grew well in the concentration range of 0–20 µg/L AFB1. Under a temperature of 20–40 °C, pH of 3.0–9.0, and anaerobic conditions, the percent removal of AFB1 was more than 60%. An analysis of the different components of DN-1 showed that cell wall adsorption was the main removal method and suggested the pathway for AFB1 removal by LAB. In addition, strain DN-1 was used as a biological preservative in artificially contaminated peanut and sunflower cakes, which significantly inhibited the growth of mold and production of AFB1. In brief, this study highlights the potential use of DN-1 as a preventive agent against aflatoxicosis via strong removal capability in the application of fermented feed or food.

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花生和向日葵蛋糕中抑霉和去除黄曲霉毒素B1的短乳杆菌DN-1的分离、鉴定和生物保存

黄曲霉毒素B1 （AFB1）是一种毒性最强的真菌毒素，广泛存在于发霉饲料中。利用生物去除方法降低AFB1已成为研究热点。本研究旨在从发霉饲料中分离出能够去除AFB1的乳酸菌，并评估其在不同环境条件下的去除能力。从饲料样品中分离到一株短乳杆菌DN-1，其AFB1去除率为71.38%。此外，DN-1对黄曲霉BNCC336156和寄生曲霉BNCC335939具有良好的抑菌活性。DN-1的最适生长温度为37℃，pH为6.0℃，在0 ~ 20µg/L AFB1浓度范围内生长良好。在温度20 ~ 40℃、pH 3.0 ~ 9.0、厌氧条件下，AFB1的去除率可达60%以上。对DN-1不同组分的分析表明，细胞壁吸附是主要的去除方法，并提出了LAB去除AFB1的途径。此外，将菌株DN-1作为生物防腐剂用于人工污染的花生和向日葵蛋糕中，可以显著抑制霉菌的生长和AFB1的产生。总之，本研究强调了DN-1在发酵饲料或食品中具有很强的去除能力，可作为黄曲霉毒素的预防剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Agriculture-Basel Agricultural and Biological Sciences-Food Science

CiteScore

4.90

自引率

13.90%

发文量

1793

审稿时长

11 weeks

期刊介绍： Agriculture (ISSN 2077-0472) is an international and cross-disciplinary scholarly and scientific open access journal on the science of cultivating the soil, growing, harvesting crops, and raising livestock. We will aim to look at production, processing, marketing and use of foods, fibers, plants and animals. The journal Agriculturewill publish reviews, regular research papers, communications and short notes, and there is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.