Use of ANCHOR viruses to validate radiative, physical and chemical decontamination systems in operational conditions.

IF 3.1 3区医学 Q1 INFECTIOUS DISEASES

Journal of Hospital Infection Pub Date : 2026-05-04 DOI:10.1016/j.jhin.2026.01.031

Franck Gallardo, Valentine Poirier, Charlotte Quentin-Froignant, Thomas Figueroa, Romain Volmer, Anais Proust, Camille Menard, Manuel Rosa-Calatrava, Morgane Mousnier, Olivier Authier, Dani Lainisalo, Pertti Lainisalo, Tero Ingelius, Marc Grandadam, Elie Marcheteau

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引用次数: 0

Abstract

Objectives: Effective disinfection is essential in settings such as high-containment laboratories, healthcare environments and public spaces, particularly for controlling the spread of pathogenic viruses. Current methods for evaluating disinfection efficacy are time-consuming, often lacking adaptability to real-world conditions.

Methods: In this study, we used ANCHOR tagged auto-fluorescent viruses enabling the detection of infectious particles by high-throughput microscopy. These models were used to mimic viral contamination and evaluate disinfection procedures, such as UV-C LED systems, self-decontaminating surface or chemical disinfection of sensitive areas such as BSL-4 showers and pressurized suits.

Results: Replicative ANCHOR-tagged viruses demonstrated high environmental persistence (particularly hAdV5 and VACV) and retained infectivity profiles suitable for disinfection studies.

Conclusions: Viral models based on ANCHOR provide a rapid (16h readout), low-risk and miniaturized tool for evaluating disinfection methods. This technology is a significant asset for early development, quality control and validation of disinfection systems under operational conditions. Albeit it cannot replace normative testing yet, this method enables the accelerated and cost-effective development of new disinfection systems.

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使用ANCHOR病毒在操作条件下验证辐射、物理和化学去污系统。

目的：有效消毒在高密闭实验室、卫生保健环境和公共场所等环境中至关重要，特别是对于控制致病性病毒的传播。目前评估消毒效果的方法耗时，往往缺乏对现实条件的适应性。方法：在本研究中，我们使用锚标记的自荧光病毒，通过高通量显微镜检测感染性颗粒。这些模型用于模拟病毒污染并评估消毒程序，例如UV-C LED系统，自净化表面或敏感区域（如BSL-4淋浴和加压服）的化学消毒。结果：复制锚标记病毒表现出高环境持久性（特别是hAdV5和VACV），并保留了适合消毒研究的传染性谱。结论：基于ANCHOR的病毒模型为评估消毒方法提供了一种快速（16小时读数）、低风险和小型化的工具。这项技术是在操作条件下消毒系统的早期开发、质量控制和验证的重要资产。尽管它还不能取代规范检测，但这种方法能够加速和经济有效地开发新的消毒系统。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Hospital Infection 医学-传染病学

CiteScore

12.70

自引率

5.80%

发文量

271

审稿时长

19 days

期刊介绍： The Journal of Hospital Infection is the editorially independent scientific publication of the Healthcare Infection Society. The aim of the Journal is to publish high quality research and information relating to infection prevention and control that is relevant to an international audience. The Journal welcomes submissions that relate to all aspects of infection prevention and control in healthcare settings. This includes submissions that: provide new insight into the epidemiology, surveillance, or prevention and control of healthcare-associated infections and antimicrobial resistance in healthcare settings; provide new insight into cleaning, disinfection and decontamination; provide new insight into the design of healthcare premises; describe novel aspects of outbreaks of infection; throw light on techniques for effective antimicrobial stewardship; describe novel techniques (laboratory-based or point of care) for the detection of infection or antimicrobial resistance in the healthcare setting, particularly if these can be used to facilitate infection prevention and control; improve understanding of the motivations of safe healthcare behaviour, or describe techniques for achieving behavioural and cultural change; improve understanding of the use of IT systems in infection surveillance and prevention and control.