In vitro evaluation of the immunostimulatory effects of PAM2CS lipopeptide-based TLR2 agonists in chicken peripheral blood mononuclear cells.

IF 2 3区农林科学 Q2 VETERINARY SCIENCES

Veterinary Research Communications Pub Date : 2026-05-07 DOI:10.1007/s11259-026-11221-4

Maramreddy Darshini, Saravanan Ramakrishnan, Firdous Ahmed, Deshkanwar S Brar, Arshpreet Kaur, Deepak B Salunke, Vishal Chander, Mithilesh Singh

{"title":"In vitro evaluation of the immunostimulatory effects of PAM2CS lipopeptide-based TLR2 agonists in chicken peripheral blood mononuclear cells.","authors":"Maramreddy Darshini, Saravanan Ramakrishnan, Firdous Ahmed, Deshkanwar S Brar, Arshpreet Kaur, Deepak B Salunke, Vishal Chander, Mithilesh Singh","doi":"10.1007/s11259-026-11221-4","DOIUrl":null,"url":null,"abstract":"<p><p>Although toll like receptor (TLR) ligands are effective vaccine adjuvants, current synthetic TLR2 agonists suffer from low solubility, high cost, and detrimental effect towards protein antigen. Present study aimed to evaluate the immunostimulatory potential of five in-house PAM2CS lipopeptide-based TLR2 agonists (P1, P2, P6, P7 and P8) using peripheral blood mononuclear cells (PBMCs) from chicken (n = 4). Each agonist (10 µg/mL) significantly upregulated the expression of interleukin (IL)-1 β and inducible nitric oxide synthase (iNOS) at 3 h post-treatment with highest expression of IL-1β mRNA was observed with P1. There was a significant up-regulation of interferon (IFN)-β and IFN-γ by treatment with P1 at 3 and 12 h intervals (p < 0.05). Further, IL-4 mRNA was significantly upregulated with P1 and P7 treatment at 12 and 24 h post-treatment respectively. Our results demonstrate that among all five agonists, P1 was the most effective in enhancing immune response genes expression including both type I and type II interferons, and in eliciting a balanced Th1 and Th2 responses.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"50 4","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Research Communications","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11259-026-11221-4","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Although toll like receptor (TLR) ligands are effective vaccine adjuvants, current synthetic TLR2 agonists suffer from low solubility, high cost, and detrimental effect towards protein antigen. Present study aimed to evaluate the immunostimulatory potential of five in-house PAM2CS lipopeptide-based TLR2 agonists (P1, P2, P6, P7 and P8) using peripheral blood mononuclear cells (PBMCs) from chicken (n = 4). Each agonist (10 µg/mL) significantly upregulated the expression of interleukin (IL)-1 β and inducible nitric oxide synthase (iNOS) at 3 h post-treatment with highest expression of IL-1β mRNA was observed with P1. There was a significant up-regulation of interferon (IFN)-β and IFN-γ by treatment with P1 at 3 and 12 h intervals (p < 0.05). Further, IL-4 mRNA was significantly upregulated with P1 and P7 treatment at 12 and 24 h post-treatment respectively. Our results demonstrate that among all five agonists, P1 was the most effective in enhancing immune response genes expression including both type I and type II interferons, and in eliciting a balanced Th1 and Th2 responses.

查看原文本刊更多论文

基于PAM2CS脂肽的TLR2激动剂对鸡外周血单核细胞免疫刺激作用的体外评价。

虽然toll样受体（TLR）配体是有效的疫苗佐剂，但目前合成的TLR2激动剂存在溶解度低、成本高、对蛋白抗原有不利影响等问题。本研究旨在利用鸡外周血单个核细胞（n = 4）评估5种基于PAM2CS脂肽的TLR2激动剂（P1、P2、P6、P7和P8）的免疫刺激潜力。各激动剂（10µg/mL）在处理后3 h显著上调白细胞介素(IL)-1 β和诱导型一氧化氮合酶（iNOS） mRNA的表达，其中P1表达量最高。在间隔3和12 h时，P1显著上调了干扰素(IFN)-β和IFN-γ的表达(p

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Veterinary Research Communications 农林科学-兽医学

CiteScore

2.50

自引率

0.00%

发文量

173

审稿时长

3 months

期刊介绍： Veterinary Research Communications publishes fully refereed research articles and topical reviews on all aspects of the veterinary sciences. Interdisciplinary articles are particularly encouraged, as are well argued reviews, even if they are somewhat controversial. The journal is an appropriate medium in which to publish new methods, newly described diseases and new pathological findings, as these are applied to animals. The material should be of international rather than local interest. As it deliberately seeks a wide coverage, Veterinary Research Communications provides its readers with a means of keeping abreast of current developments in the entire field of veterinary science.