Trypanosomatidae (Kinetoplastea: Trypanosomatida) diversity infecting the South American coati Nasua nasua (Carnivora: Procyonidae) in urban forest fragments in the Brazilian Midwest.

IF 4.8 2区医学 Q2 IMMUNOLOGY

Frontiers in Cellular and Infection Microbiology Pub Date : 2026-04-22 eCollection Date: 2026-01-01 DOI:10.3389/fcimb.2026.1822974

Gabriel Carvalho de Macedo, Wanessa Gomes Teixeira Barreto, Carina Elisei de Oliveira, Leonardo França do Nascimento, Andreza Castro Rucco, Julia Gindri Bragato Pistori, William Oliveira de Assis, Geovanna Silva Dos Santos, Wesley Arruda Gimenes Nantes, Gisele Braziliano de Andrade, André Luiz Rodrigues Roque, Heitor Miraglia Herrera

{"title":"Trypanosomatidae (Kinetoplastea: Trypanosomatida) diversity infecting the South American coati Nasua nasua (Carnivora: Procyonidae) in urban forest fragments in the Brazilian Midwest.","authors":"Gabriel Carvalho de Macedo, Wanessa Gomes Teixeira Barreto, Carina Elisei de Oliveira, Leonardo França do Nascimento, Andreza Castro Rucco, Julia Gindri Bragato Pistori, William Oliveira de Assis, Geovanna Silva Dos Santos, Wesley Arruda Gimenes Nantes, Gisele Braziliano de Andrade, André Luiz Rodrigues Roque, Heitor Miraglia Herrera","doi":"10.3389/fcimb.2026.1822974","DOIUrl":null,"url":null,"abstract":"Introduction: South American coatis are reported as key-hosts of trypanosomatid species in Brazilian Pantanal, presenting high parasitemia and infection rates, long-lasting infections, ability to bioaccumulate Trypanosoma evansi, T. rangeli and different genotypes of T. cruzi, and biological features that favor these parasites' transmission. Moreover, this mammal species was also reported parasitized by the monoxenous trypanosomatid Crithidia mellificae and included in the epidemiological scenario of Visceral Leishmaniasis in Campo Grande (CG), Brazilian Midwest. Within this context, the aim of this work was to assess the diversity of trypanosomatid species in coatis from CG.Methods: Samples of 110 South American coatis captured in two forest fragments were submitted to Nested Polymerase Chain Reaction (nPCR) of small ribosomal ribonucleic acid subunit (SSU rRNA) gene and Sanger sequencing analyses.Results: The positivity rate of nPCR was 36.4% (40/110) among individuals, including infections by Leishmania infantum, L. amazonensis, T. cruzi and the Molecular Operational Taxonomic Unit Trypanosomatidae sp. CROT. When combining SSU rRNA detection and sequencing with previous published data from the same coati cohort, we observed a positivity rate of 47.3% (52/110), in single (36.4%; n = 40/110) or mixed Trypanosomatidae infections (10.9%; n = 12/110).Discussion: Our findings indicate the South American coati is a key-hosts species in the ecology of trypanosomatids also in the urban environment of CG.","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"16 ","pages":"1822974"},"PeriodicalIF":4.8000,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13143924/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Cellular and Infection Microbiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3389/fcimb.2026.1822974","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"IMMUNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction: South American coatis are reported as key-hosts of trypanosomatid species in Brazilian Pantanal, presenting high parasitemia and infection rates, long-lasting infections, ability to bioaccumulate Trypanosoma evansi, T. rangeli and different genotypes of T. cruzi, and biological features that favor these parasites' transmission. Moreover, this mammal species was also reported parasitized by the monoxenous trypanosomatid Crithidia mellificae and included in the epidemiological scenario of Visceral Leishmaniasis in Campo Grande (CG), Brazilian Midwest. Within this context, the aim of this work was to assess the diversity of trypanosomatid species in coatis from CG.

Methods: Samples of 110 South American coatis captured in two forest fragments were submitted to Nested Polymerase Chain Reaction (nPCR) of small ribosomal ribonucleic acid subunit (SSU rRNA) gene and Sanger sequencing analyses.

Results: The positivity rate of nPCR was 36.4% (40/110) among individuals, including infections by Leishmania infantum, L. amazonensis, T. cruzi and the Molecular Operational Taxonomic Unit Trypanosomatidae sp. CROT. When combining SSU rRNA detection and sequencing with previous published data from the same coati cohort, we observed a positivity rate of 47.3% (52/110), in single (36.4%; n = 40/110) or mixed Trypanosomatidae infections (10.9%; n = 12/110).

Discussion: Our findings indicate the South American coati is a key-hosts species in the ecology of trypanosomatids also in the urban environment of CG.

查看原文本刊更多论文

巴西中西部城市森林碎片中南美coati Nasua Nasua（食肉目：原虫科）的锥虫科（Kinetoplastea: Trypanosomatida）多样性

简介：据报道，南美长鼻浣熊是巴西潘塔纳尔地区锥虫的主要宿主，具有较高的寄生虫血症和感染率，感染时间长，具有生物蓄积伊文氏锥虫、朗格里锥虫和不同基因型克氏锥虫的能力，以及有利于这些寄生虫传播的生物学特征。此外，巴西中西部Campo Grande （CG）的内脏利什曼病（内脏利什曼病）流行病学情景中也报道了该哺乳动物被单毒锥虫Crithidia mellificae寄生。在此背景下，本工作的目的是评估锥虫物种的多样性，从羽衣从CG。方法：采用巢式聚合酶链反应（Nested Polymerase Chain Reaction, nPCR）对在两个森林片段中捕获的110只南美长鼻猴进行小核糖体核糖核酸亚基（SSU rRNA）基因的分析和Sanger测序。结果：nPCR检测阳性率为36.4%(40/110)，检测对象包括婴幼儿利什曼原虫、亚马逊乳杆菌、克氏锥虫和分子操作分类单位锥虫科。将SSU rRNA检测和测序与先前发表的来自同一coati队列的数据相结合，我们观察到阳性率为47.3%(52/110)，单个（36.4%,n = 40/110）或混合锥虫病感染（10.9%,n = 12/110）。讨论：我们的研究结果表明，南美洲coati是锥虫生态中的关键宿主物种，也是CG城市环境中的关键宿主物种。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Frontiers in Cellular and Infection Microbiology IMMUNOLOGY-MICROBIOLOGY

CiteScore

7.90

自引率

7.00%

发文量

1817

审稿时长

14 weeks

期刊介绍： Frontiers in Cellular and Infection Microbiology is a leading specialty journal, publishing rigorously peer-reviewed research across all pathogenic microorganisms and their interaction with their hosts. Chief Editor Yousef Abu Kwaik, University of Louisville is supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide. Frontiers in Cellular and Infection Microbiology includes research on bacteria, fungi, parasites, viruses, endosymbionts, prions and all microbial pathogens as well as the microbiota and its effect on health and disease in various hosts. The research approaches include molecular microbiology, cellular microbiology, gene regulation, proteomics, signal transduction, pathogenic evolution, genomics, structural biology, and virulence factors as well as model hosts. Areas of research to counteract infectious agents by the host include the host innate and adaptive immune responses as well as metabolic restrictions to various pathogenic microorganisms, vaccine design and development against various pathogenic microorganisms, and the mechanisms of antibiotic resistance and its countermeasures.