Multiplexed immunochromatographic strip based on multicolor fluorescent microspheres for simultaneous detection of three herbicides

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2026-05-09 DOI:10.1007/s00604-026-08045-z

Hui Cao, Jie Chen, Li Zhao, Chenxu Yan, Lin Ma, Jinsong Yu, Xiuxiu Wu, Fei Xu

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Abstract

A novel multiplex immunochromatographic assay (ICA) based on quantum-dot-encoded multicolor fluorescent microsphere (MFMs) was developed for the simultaneous and quantitative detection of prometryn (PRO), diquat (DQ), and sulfometuron-methyl (SMR). Red, yellow, and green MFMs were fabricated by embedding CdSe/ZnS QDs into polymer microspheres and subsequently conjugated with specific monoclonal antibodies, generating highly uniform MFM-mAb probes with clear and stable emission signals. The multiplex ICA exhibited excellent analytical performance, with limits of detection of 0.41 ng/mL for PRO, 9.36 ng/mL for DQ, and 1.32 ng/mL for SMR, better than most ELISA-based assays. The strip also demonstrated high specificity, good storage stability, and reliable performance in complex matrices. In spiked river water and vegetable samples, recoveries ranged from 85.53% to 109.67% with RSDs of 1.33–7.02%, indicating strong applicability. Overall, this MFM-ICA provides an effective platform for rapid multi-herbicide screening.

Graphical Abstract

The alternative text for this image may have been generated using AI.

查看原文本刊更多论文

基于多色荧光微球的多重免疫层析条同时检测三种除草剂

建立了一种基于量子点编码多色荧光微球（MFMs）的多重免疫层析（ICA）方法，用于同时定量检测prometryn （PRO）、diquat （DQ）和磺美敦酮-甲基（SMR）。通过将CdSe/ZnS量子点包埋在聚合物微球中，并与特异性单克隆抗体偶联制备红、黄、绿色mfs - mab探针，制备出高度均匀的mfs - mab探针，发射信号清晰稳定。多重ICA具有良好的分析性能，PRO的检出限为0.41 ng/mL， DQ的检出限为9.36 ng/mL， SMR的检出限为1.32 ng/mL，优于大多数elisa法。该试纸条具有高特异性、良好的储存稳定性和在复杂基质中的可靠性能。在加标后的河水和蔬菜样品中，加标回收率为85.53% ~ 109.67%，rsd为1.33 ~ 7.02%，适用性强。综上所述，该方法为多种除草剂的快速筛选提供了有效的平台。此图像的替代文本可能是使用AI生成的。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.