Cynandione A improves white adipose tissue homeostasis in high-fat diet-fed mice.

IF 3.2 4区医学 Q2 PHARMACOLOGY & PHARMACY

Journal of Pharmacy and Pharmacology Pub Date : 2026-05-02 DOI:10.1093/jpp/rgag038

Atsushi Sawamoto, Misaki Shiba, Satoshi Okuyama, Chan Jae Cho, Jae Hyun Kim, Mitsunari Nakajima

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引用次数: 0

Abstract

Objectives: Cynandione A (CA), a major bioactive compound isolated from Cynanchum wilfordii Radix, is a crude drug traditionally used in East Asia. We have previously demonstrated that CA induces a beige adipocyte-like phenotype in vitro. This study aimed to investigate the in vivo effects of CA on white adipose tissue (WAT) function.

Methods: C57BL/6 J mice were fed a high-fat diet (HFD) and administered CA (5 or 15 mg/kg, intraperitoneally, once daily) for eight weeks. Body weight, glucose tolerance, insulin sensitivity, and serum parameters were evaluated. Histological analyses of WAT and liver were performed, and gene and protein expression related to beige adipocyte features and mitochondrial biogenesis were assessed in inguinal WAT (iWAT).

Key findings: CA treatment did not affect body weight, glucose tolerance, insulin sensitivity, or serum parameters. However, adipocyte size was significantly reduced in inguinal and epididymal WAT in mice treated with CA at 15 mg/kg (43.8% reduction, P < .001; 33.1% reduction, P = .021, respectively). Moreover, CA increased the expression of beige adipocyte-specific genes (Tmem26, 2.8-fold, P < .001; Cd137, 3.8-fold, P < .001) and mitochondrial marker proteins (UCP1, 2.1-fold, P = .007; TOM20, 2.2-fold, P < .001; VDAC, 1.6-fold, P = .019) in iWAT.

Conclusions: CA modulates WAT plasticity and improves WAT homeostasis in HFD-fed mice.

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Cynandione A改善高脂肪喂养小鼠的白色脂肪组织稳态。

目的：Cynandione A （CA）是一种东亚传统药材，是一种从Cynanchum wilfordii Radix中分离得到的主要生物活性化合物。我们之前已经证明CA在体外诱导米色脂肪细胞样表型。本研究旨在探讨CA对白色脂肪组织（WAT）功能的体内影响。方法：C57BL/6 J小鼠饲喂高脂饲料（HFD），同时给予CA（5或15 mg/kg，腹腔注射，每日1次），连续8周。评估体重、葡萄糖耐量、胰岛素敏感性和血清参数。对WAT和肝脏进行组织学分析，并在腹股沟WAT （iWAT）中评估与米色脂肪细胞特征和线粒体生物发生相关的基因和蛋白表达。主要发现：CA治疗不影响体重、葡萄糖耐量、胰岛素敏感性或血清参数。然而，15 mg/kg剂量的CA显著降低了小鼠腹股沟和附睾WAT的脂肪细胞大小（减少43.8%），P结论：CA调节了hfd喂养小鼠WAT的可塑性，改善了WAT的稳态。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Pharmacy and Pharmacology 医学-药学

CiteScore

6.60

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： JPP keeps pace with new research on how drug action may be optimized by new technologies, and attention is given to understanding and improving drug interactions in the body. At the same time, the journal maintains its established and well-respected core strengths in areas such as pharmaceutics and drug delivery, experimental and clinical pharmacology, biopharmaceutics and drug disposition, and drugs from natural sources. JPP publishes at least one special issue on a topical theme each year.