Analysis of the Composition and Phylogenetic Relationships of the Acanthosaura coronata Complex Including Molecular Identification of Historical Specimens.

IF 2.7 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animals Pub Date : 2026-04-20 DOI:10.3390/ani16081261

Natalia B Ananjeva, Maryia I Matsiushova, Anton O Svinin, Olga S Bezman-Moseyko, Luan Nguyen Thanh, Nikolai L Orlov

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引用次数: 0

Abstract

The genus Acanthosaura is characterized by a high level of cryptic species diversity and is subdivided into several species complexes. The phylogenetic relationships within the A. coronata complex remain unresolved due to the presence of cryptic lineages and limited molecular data for several species. In this study, these relationships are clarified using a molecular genetic analysis that integrates newly collected field samples and historical museum specimens with previously uncertain identification. Three mitochondrial genes (cyt b, COI, and ND2) from samples, including fresh collections of A. murphyi from Phu Yen Province (Vietnam) and museum specimens from Vietnam and Myanmar, were analyzed. In addition, morphological characters of the examined specimens with diagnostic traits of known species were compared. Phylogenetic analyses confirmed the distinct species status of A. murphyi and enabled the taxonomic reassignment of previously undetermined museum specimens to this species. Specimens from Vietnam and Myanmar formed a single, well-supported clade, suggesting a broader distribution for A. murphyi than previously recognized. It is demonstrated for the first time that A. murphyi belongs to the A. coronata complex, together with A. coronata and A. cuongi, a result consistently supported by both genetic distances and phylogenetic tree topology.

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冠棘龙复合体的组成及系统发育关系分析及历史标本的分子鉴定。

棘龙属的特点是具有高度的隐物种多样性，并被细分为几个物种复合体。由于存在隐伏的谱系和有限的分子数据，冠状一枝内的系统发育关系仍未得到解决。在这项研究中，这些关系是通过分子遗传学分析来澄清的，该分析将新收集的现场样本和历史博物馆标本与以前不确定的鉴定结合起来。研究人员分析了来自越南富延省（Phu Yen Province）新收集的A. murphyi标本以及越南和缅甸博物馆标本的三个线粒体基因（cyt b、COI和ND2）。此外，还将检测标本的形态特征与已知种的诊断特征进行了比较。系统发育分析证实了A. murphyi的独特物种地位，并使以前未确定的博物馆标本的分类重新分配到该物种。来自越南和缅甸的标本形成了一个单一的、支持良好的分支，表明a . murphyi的分布比以前认识到的更广泛。该研究首次证明了a . murphyi与a . coronata和a . cuongi属于a . coronata复合体，遗传距离和系统发育树拓扑结构一致支持这一结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Animals Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

4.90

自引率

16.70%

发文量

3015

审稿时长

20.52 days

期刊介绍： Animals (ISSN 2076-2615) is an international and interdisciplinary scholarly open access journal. It publishes original research articles, reviews, communications, and short notes that are relevant to any field of study that involves animals, including zoology, ethnozoology, animal science, animal ethics and animal welfare. However, preference will be given to those articles that provide an understanding of animals within a larger context (i.e., the animals'' interactions with the outside world, including humans). There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental details and/or method of study, must be provided for research articles. Articles submitted that involve subjecting animals to unnecessary pain or suffering will not be accepted, and all articles must be submitted with the necessary ethical approval (please refer to the Ethical Guidelines for more information).