Comparative Analysis of Conventional and Digital Microscopy for Counting Mitotic Figures in Cutaneous Neoplasms of Dogs and Cats.

IF 2.7 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animals Pub Date : 2026-04-21 DOI:10.3390/ani16081268

Larissa G A Moreira, Lucas R Souza, Nayara F Paula, Taismara S Oliveira, Ayisa R Oliveira, Taryn A Donovan, Christof A Bertram, Tatiane A Paixão, Renato L Santos

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Abstract

The use of digitized slides for histopathological diagnosis has become common in veterinary pathology, and the validation of diagnostic techniques that are extrapolated from the evaluation of glass slides is needed. The goal of this study is to evaluate the efficiency of counting mitotic figures in physical glass slides and digitized slides of cutaneous tumors of dogs and cats. The mitotic count was performed by three pathologists on glass and digitized slides of ninety skin tumors, including 30 squamous cell carcinomas in dogs and cats, 30 mast cell tumors and 30 soft tissue tumors in dogs. An additional assessment of cellular proliferation was performed with immunohistochemistry for Ki67. Spearman's correlation for the mean count of mitotic figures between the three observers on physical and digitized slides demonstrated a strong positive correlation for squamous cell carcinomas and mesenchymal tumors and a moderate correlation for mast cell tumors. Inter-observer agreement was moderate between the two methods. In conclusion, the results found suggest that digitized slides can be used reliably for mitotic figure counting in cutaneous neoplasms in small animals, without compromising their classification or prediction of prognosis.

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犬、猫皮肤肿瘤有丝分裂象的传统显微镜与数字显微镜比较分析。

使用数字化载玻片进行组织病理学诊断在兽医病理学中已经变得很普遍，并且需要对从玻璃载玻片的评估中推断出来的诊断技术进行验证。本研究的目的是评估在狗和猫皮肤肿瘤的物理玻片和数字化玻片上计数有丝分裂图的效率。有丝分裂计数由三名病理学家在90个皮肤肿瘤的玻片和数字化玻片上进行，其中包括30个狗和猫的鳞状细胞癌，30个肥大细胞瘤和30个狗的软组织肿瘤。用免疫组织化学对Ki67进行细胞增殖的额外评估。三个观察者在物理和数字化玻片上的有丝分裂图的平均计数的Spearman相关性表明，鳞状细胞癌和间充质瘤与肥大细胞瘤有很强的正相关性。两种方法之间的观察者之间的一致性是中等的。综上所述，本研究结果表明，数字化切片可以可靠地用于小动物皮肤肿瘤的有丝分裂象计数，而不会影响其分类或预测预后。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Animals Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

4.90

自引率

16.70%

发文量

3015

审稿时长

20.52 days

期刊介绍： Animals (ISSN 2076-2615) is an international and interdisciplinary scholarly open access journal. It publishes original research articles, reviews, communications, and short notes that are relevant to any field of study that involves animals, including zoology, ethnozoology, animal science, animal ethics and animal welfare. However, preference will be given to those articles that provide an understanding of animals within a larger context (i.e., the animals'' interactions with the outside world, including humans). There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental details and/or method of study, must be provided for research articles. Articles submitted that involve subjecting animals to unnecessary pain or suffering will not be accepted, and all articles must be submitted with the necessary ethical approval (please refer to the Ethical Guidelines for more information).