Components with potential anti-inflammatory effect from the seeds of Dolichos lablab

Abstract

Four new nitrogenous compounds, designated as dolilabnitrogenousols A₁−A₃, B (1−4), and six new others, namely dolilabnosides A−C, D₁, D₂ (5−9), and (2S)-[[(3-furanyl)carbonyl]oxy]butanedioic acid (10), along with ten known compounds (11–20), which were initially isolated from Lablab genus, were obtained from the seeds Dolichos lablab L., a plant with medicine food homology. Their structures were determined based on high resolution electrospray ionization MS (HR-ESI-MS) data, various NMR spectroscopic analyses, electronic circular dichroism (ECD) calculations, as well as chemical reactions. Moreover, the expression level of nitric oxide (NO) was measured using a lipopolysaccharide (LPS)-induced RAW264.7 cell model to evaluate the anti-inflammatory activity of the obtained compounds. The results of bioactive assay indicated that compounds 1–4, 6, 7, 10–13, 16, 17, and 20 displayed significant inhibitory activity on NO release in RAW264.7 cells in a dose-dependent manner. Additionally, the structure-activity relationships were summarized. These findings highlight the potential of the ingredients from D. lablab seeds in the treatment of inflammation-related disorders.

Graphical abstract